摘要
【目的】探究娇小白掌的组培快繁体系,为工厂化生产提供技术参考。【方法】以娇小白掌新生顶芽为外植体进行组织培养研究。【结果】娇小白掌适宜的不定芽诱导培养基为MS+2.0mg/L6-BA+0.1mg/L NAA,诱导率100%,诱导丛生芽数量4~5个;适合的继代增殖培养基为MS+1.0mg/L6-BA+0.3mg/L NAA和MS+0.5mg/L6-BA+0.3mg/LNAA交替使用,增殖系数达3.5,芽健壮,可直接用于生根培养;最佳生根培养基为MS+1000mg/LAC,生根率100%,根数3~4,根长30mm左右,种植成活率95%以上。【结论】初步构建了娇小白掌的组培快繁体系,优化了白掌组培技术,能够实现娇小白掌的高效产业化生产。
[Objective] The objective of this study is to explore the tissue culture and rapid propagation system of Spathiphyllum‘Petite',and to provide references for industrial production.[Method]In this paper,the new terminal bud of Spathiphyllum‘Petite'was used as explants for tissue culture.[Result]The suitable medium for adventitious bud induction was MS medium+2.0 mg/L 6-BA+0.1 mg/L NAA,the induction rate was 100%,and the number of cluster buds was 4~5.The suitable proliferation media were MS medium+1.0 mg/L 6-BA+0.3 mg/L NAA and MS medium+0.5 mg/L 6-BA+0.3 mg/L NAA,which were used alternately in the subculture process,the proliferation coefficient reached 3.5,and the buds were robust which could be directly used for rooting culture.The optimal rooting medium was MS medium+1000 mg/L AC,the rooting rate was 100%,the number of root was 3-4,the root length was about 30 mm,and the survival rate of rooting seedlings was above 95%.[Conclusion]The tissue culture and rapid propagation system of Spathiphyllum‘Petite'was preliminarily established,and its tissue culture technology was optimized.It could realize the efficient industrial production of Spathiphyllum‘Petite'.
作者
王文平
简丽观
陈海燕
李雪
Wang Wenping;Jian Liguan;Chen Haiyan;Li Xue(Sunshine Horticulture company,Quanzhou,Fujian 362012,China)
出处
《东南园艺》
2023年第3期161-167,共7页
Southeast Horticulture
基金
福建省区域发展项目(2020N3007)。
关键词
娇小白掌
顶芽
组织培养
Spathiphyllum‘Petite'
Terminal bud
Tissue culture
