摘要
为了确定苦参碱和氧化苦参碱对奶牛子宫内膜炎致病菌的体外抑菌活性,选取奶牛子宫内膜炎致病菌大肠埃希菌和金黄色葡萄球菌为供试菌株,采用纸片扩散法、牛津杯法对2种生物碱进行了药敏试验,采用液体倍比稀释法测定了其最小抑菌浓度(MIC)。结果表明,苦参碱和氧化苦参碱对奶牛子宫中分离的耐药性大肠埃希菌和耐甲氧西林金黄色葡萄球菌(MRSA)均有一定的抑制作用。苦参碱对耐药性大肠埃希菌和MRSA的MIC分别为12.5 mg/mL和25 mg/mL,氧化苦参碱对耐药性大肠埃希菌和MRSA的MIC分别为25mg/mL和50mg/mL。
To investigate the antibacterial effects of matrine and oxymatrine on the pathogenic bacteria isolated from uterus of cows with endometritis,the experiment was conducted in vitro.The antibacterial effects on drug-resistanct E.coli and methicillin-resistant Staphylococcus aureus （MRSA） were screened by Kirby-Bauer paper dispersion and Oxford cup method respectively. The minimal inhibitory concentration （MIC） of matrine and oxymatrine against drug-resistanct E.coli and MRSA were determined respectively by the method of tube double dilution method.The results showed the drug-resistanct E.coli and MRSA were inhibited by matrine and oxymatrine in different degree.MIC of the matrine against the drug-resistanct E.coli and MRSA were 12.5 mg/mL and 25 mg/mL.MIC of the oxymatrine against the drug-resistanct E.coli and MRSA were 25 mg/mL and 50 mg/mL.
出处
《动物医学进展》
北大核心
2017年第1期65-68,共4页
Progress In Veterinary Medicine
基金
宁夏自然科学基金项目(NZ12202)
