摘要
目的了解宫内生长受限(IUGR)胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β表达水平,探讨孕鼠补充牛磺酸对其表达水平的影响及临床意义。方法采用妊娠期全程饥饿法建立IUGR胎鼠模型,将15只孕鼠随机分为3组:正常对照组、IUGR组、IUGR+补充牛磺酸组[300mg/(kg·d)](牛磺酸组)。采用酶联免疫吸附法测定三组胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的含量。结果对照组、IUGR组、牛磺酸组胎鼠脑组织IL-6含量分别为:(81.070±5.286)pmol/L、(148.362±18.733)pmol/L和(107.361±11.184)pmol/L,差异有统计学意义(H=43.760,P=0.00);三组胎鼠脑组织IL-8含量分别为:(90.573±7.344)pmol/L、(165.596±8.635)pmol/L和(126.309±11.008)pmol/L,差异有统计学意义(H=45.910,P=0.00);三组胎鼠脑组织TNF-α含量分别为:(8.231±0.607)pmol/L、(14.881±0.703)pmol/L和(11.709±0.936)pmol/L,差异有统计学意义(H=45.929,P=0.00);三组胎鼠脑组织IL-1β含量分别为:(46.693±4.122)pmol/L、(84.799±4.315)pmol/L和(62.407±4.631)pmol/L,差异有统计学意义(H=46.064,P=0.00)。结论孕鼠补充牛磺酸可减少IUGR胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的表达。
Objectives To explore the effects and clinical significance of prenatal taurine on IL-6,IL-8,TNF-a and IL- 1β in fetal rat brains of intrauterine growth restriction（IUGR）. Methods IUGR was induced by food restriction throughout pregnancy. Pregnant rats were randomly divided into 3 groups： control group, IUGR group and taurine group [300 mg/（kg · d）]. The expressions of serum IL-6, IL-8 ,TNF-α and IL-1β in brains were detected by Enzyme linked immunosorbent- assay（ELlSA）. Results The expressions of IL-6 in control group,test group and taurine group were： （81. 070 ± 5. 286） pmol/L, （ 148. 362 ± 18. 733） pmol/L and （ 107. 361 ±11. 184） pmol/L, there were significant differences among the three groups （H=43. 760,P= 0.00）. The contents of IL-8 in three groups were： （90. 573 ±7. 344）pmol/L, （165. 596 ±8. 635） pmol/L and （ 126. 309 ± 11. 008） pmol/L. There were significant differences among the three groups （ H = 45.910, P = 0.00）. The expressions of TNF-α in three groups were： （8. 231±0.607）pmol/L, （14. 881± 0. 703）pmol/L and （11. 709± 0. 936）pmol/L,there were significant differences among the three groups （H=45. 929 ,P=0. 00）. The expressions of IL-1β in three groups were： （46. 693±4. 122）pmol/L, （84. 799±4. 315）pmol/L and （62. 407±4. 631）pmol/L,there were significant differences among the three groups （H=46. 064,P=0.00）. Conclusion Prenatal taurine can reduce expressions IL- 6 ,IL-8 ,TNF-αand IL-1β in fetal rat brains of IUGR.
出处
《中国儿童保健杂志》
CAS
2017年第1期28-31,共4页
Chinese Journal of Child Health Care
基金
国家自然科学基金资助项目(81471087)
