摘要
背景:骨髓间充质干细胞在体外经5-氮胞苷诱导后可分化为心肌样细胞这一研究成果使细胞移植治疗心脏疾病成为可能。目的:探讨不同浓度5-氮胞苷在大鼠骨髓间充质干细胞向心肌细胞分化中的诱导作用。方法:取第3代骨髓间充质干细胞,分别采用含0,5,10,20μmol/L 5-氮胞苷的DMEM培养液孵育24 h,去除诱导培养液,用含体积分数为5%胎牛血清的LG-DMEM培养28 d,采用免疫细胞化学方法检测心肌肌钙蛋白I表达。结果与结论:(1)第3代骨髓间充质干细胞经5-氮胞苷诱导24 h后出现细胞死亡现象。5,10μmol/L 5-氮胞苷组细胞死亡相对较少,20μmol/L 5-氮胞苷组细胞死亡较多;(2)5μmol/L 5-氮胞苷组肌钙蛋白Ⅰ染色阳性率显著低于10μmol/L 5-氮胞苷组和20μmol/L 5-氮胞苷组(P<0.05),10μmol/L和20μmol/L 5-氮胞苷组肌钙蛋白Ⅰ阳性率差异无显著性意义(P>0.05);(3)结果表明,5-氮胞苷可以在体外诱导大鼠骨髓间充质干细胞向心肌样细胞分化,且10μmol/L 5-氮胞苷诱导效果较好。
BACKGROUND：Bone marrow mesenchymal stem cel s can be differentiated into myocardial cel s induced by 5-azacytidine in vitro, which provides an opportunity for cel transplantation in the treatment of heart disease. OBJECTIVE：To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cel s in rats. METHODS：Passage 3 bone marrow mesechymal stem cel s were incubated with DMEM containing 0, 5, 10, 20μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5%fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method. RESULTS AND CONCLUSION：Cel death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20μmol/L group than the 5 and 10μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5μmol/L group than the 10 and 20μmol/L groups （P〈0.05）, but there was no significant difference between the 10 and 20μmol/L groups （P〉0.05）. These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cel s into myocardial cel s in vitro, and its optimal concentration is 10μmol/L.
作者
贾秀丽
李昌平
Jia Xiu-ti Li Chang-ping(Dazhou Vocational and Technical College, Dazhou 635000, Sichuan Province, China Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2016年第50期7469-7474,共6页
Chinese Journal of Tissue Engineering Research
基金
西南医科大学附属医院基金资助项目(2014-03)~~
