摘要
目的建立高效液相色谱法测定跌打活血酊中羟基红花黄色素A和阿魏酸的含量。方法使用ODS2 C_(18)(4.6 mm×150 mm,5μm)色谱柱,其中羟基红花黄色素A的色谱条件:以甲醇-0.7%磷酸溶液(25∶75)为流动相,流速为1 m L·min^(-1),检测波长为403 nm,柱温为30℃,进样量为10μL;阿魏酸的色谱条件:以乙腈-0.085%磷酸溶液(17∶83)为流动相,流速为1 m L·min^(-1),检测波长为316 nm,柱温为35℃,进样量为10μL。结果羟基红花黄色素A在0.0478~2.39μg与峰面积线性关系良好(r^2=0.9996),样品的平均加样回收率为98.6%;阿魏酸在0.028 35~2.835μg与峰面积线性关系良好(r^2=0.9998),样品的平均加样回收率为99.1%。结论建立的方法具有操作简便、结果准确、灵敏度高等优点,可用于控制跌打活血酊的质量。
Objective To establish an HPLC method to determine the content of hydroxy safflower yellow A and ferulic acid in Dieda Huoxue tincture. Methods ODS2 C18（4.6 mm×150 mm, 5 μm） was used as chromatographic column. Chromatographic conditions for hydroxy safflower yellow A： methanol-0.7% phosphoric acid solution（25∶75） as the mobile phase, with the flow rate 1 m L·min^-1, detection wavelength 403 nm, column temperature 30 ℃, and injection volume 10 μL. Chromatographic conditions for ferulic acid： acetonitrile-0.085% phosphoric acid solution（17∶83） as the mobile phase, with the flow rate 1 m L·min^-1, detection wavelength 316 nm, column temperature 35 ℃, and injection volume 10 μL. Results Hydroxy safflower yellow A had a good linearity at 0.0478- 2.39 μg（r^2= 0.9996）, and the average recovery was 98.6%. Ferulic acid had a good linearity at 0.028 35- 2.835 μg（r^2= 0.9998）, and the average recovery was 99.1%. Conclusion The method is simple, accurate, and highly sensitive, and can be used for the quality control of Dieda Huoxue tincture.
作者
熊婷
封传华
李刚
张静
徐兰
陶晓璇
XIONG Ting FENG Chuan-hua LI Gang ZHANG Jing XU Lan TAO Xiao-xuan(Department of Pharmacy, No.94 Hospital of the People＇s Liberation Army, Nanchang 33000)
出处
《中南药学》
CAS
2016年第12期1365-1368,共4页
Central South Pharmacy
