摘要
目的建立高效液相色谱法同时测定复方中药制剂中芍药苷、甘草酸和丹参酮ⅡA的含量。方法样品经50%乙醇超声提取,经Alltima-C18柱分离,以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱,采用可变波长检测器（variable wavelength detector,VWD）在250 nm波长处进行检测。结果芍药苷、甘草酸和丹参酮ⅡA能实现较好的分离,在各自线性范围内线性关系良好;平均回收率为97.5-102.4%,RSD〈2.0%。结论该方法操作快速简便、精密度和重复性好,可适用于复方中药制剂中芍药苷、甘草酸和丹参酮ⅡA的同时测定。
Objective To establish a method for simultaneous determination of paeoniflorin,glycyrrhizic acid and tanshinone II A in compound traditional Chinese medicine preparation by high performance liquid chromatography（HPLC）.Methods Samples were ultrasonically extracted with 50%ethanol,separated by Alltima-C_（18） column with acetonitrile-0.05%phosphoric acid solution as the mobile phase for gradient elution,and then detected by variable wavelength detector（VWD） at the wavelength of 250 nm.Results Paeoniflorin,glycyrrhizic acid and tanshinone II A could be completely separated,and had good linear relationships in their linear ranges.The average recoveries were 97.5%-102.4%with RSDs 2.0%.Conclusion The method is rapid,simple,accurate and repeatable,which can be used for the simultaneous determination of paeoniflorin,glycyrrhizic acid and tanshinone II A in compound traditional Chinese medicine preparation.
出处
《食品安全质量检测学报》
CAS
2016年第10期4203-4209,共7页
Journal of Food Safety and Quality
关键词
芍药苷
甘草酸
丹参酮ⅡA
高效液相色谱法
paeoniflorin
glycyrrhizic acid
tanshinone Ⅱ A
high performance liquid chromatography
