摘要
目的:建立超高效液相色谱法(UPLC)同时测定银翘散中绿原酸、连翘苷、连翘酯苷A、牛蒡苷含量的方法。方法:采用UPLC法,ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),乙腈-0.2%磷酸水溶液梯度洗脱,流速0.3 mL·min^(-1),检测波长230 nm,柱温为40℃,同时测定4种指标性成分的含量。结果:绿原酸、连翘酯苷A、连翘苷、牛蒡苷分别在10.75~344.0,7.062 5~226.0,2.375~76.0,9.656 25~309.0μg·mL^(-1)线性关系良好(r≥0.999 1)。结论:该方法操作简便快速,结果准确,专属性强,重复性好,可用于银翘散的质量控制。
OBJECTIVE To establish a UPLC method for simultaneous determination of chlorogenic acid,forsythiaside A,forsythin and arctiin in Yinqiao Powder.METHODS UPLC method was adopted,with ACQUITY UPLC BEH C_（18） chromatographic column（2.1 mm×100 mm,1.7μm）,gradient elution of acetonitril e-0.2% phosphoric acid for gradient elution at flow rate of 0.3 mL·min^（-1）,detection wavelength at 230 nm,column temperature at 40 ℃.Contents of 4 index components were measured simultaneously.RESULTS Chlorogenic acid,forsythin A,forsythin and arctiin had good linear relationship in the ranges of 10.75-344.0,7.062 5-226.0,2.375-76.0and 9.656 25-309.0μg·mL^（-1）,respectively.CONCLUSIONThe method is simple,quick,accurate,with strong specificity and good repeatability,and can be used to control the quality of Yinqiao Powder.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第24期2170-2172,共3页
Chinese Journal of Hospital Pharmacy
基金
贵州省科技成果转化引导基金计划项目(编号:黔科合成转字[2015]5202)
