摘要
苯丙烷途径是植物体内广泛存在的次生代谢途径,此代谢过程中的第1个关键酶为苯丙氨酸解氨酶(Phenylalanine ammonia-1yase,PAL)。苯丙氨酸解氨酶通过对基因的起始转录、频率的控制和对外界诱导因子的响应来调控基因的表达,并且其表达水平对于下游黄酮类物质的合成起着非常重要的作用。在一定条件下,苯丙氨酸解氨酶可利用其特殊的酶学性质逆向催化生产L-苯丙氨酸。通过一系列的生物信息学分析,确定拟南芥中苯丙氨酸解氨酶的存在,并进行基因的克隆及表达分析,为L-苯丙氨酸的生产提供新的物种来源。
Phenylalanine ammonia-lyase（PAL）is the first key enzyme involved in the metabolism of phenylpropanoid, which is a widespread metabolic pathway in plants. Phenylalanine ammonia-lyase（PAL）regulates the expression of genes by transcription initiation,frequency control and response to external factors, and its expression level plays an important role in downstream flavonoid synthesis.Under certain conditions, phenylalanine ammonia-lyase can be used to reverse-catalyze the production of L-phenylalanine by its special enzymatic properties. In this paper, the presence of phenylalanine ammonia-lyase in Arabidopsis thaliana was determined by a series of bioinformatics analysis, and cloning and expression of the gene analysis, for the production of L-phenylalanine to provide a new species source.
出处
《山西农业科学》
2016年第12期1767-1771,共5页
Journal of Shanxi Agricultural Sciences
关键词
拟南芥
苯丙氨酸解氨酶
生物信息学
克隆
Arabidopsis thaliana
phenylalanine ammonia-lyase
bioinformatics
clone
