摘要
目的观察吡哆胺对动脉粥样硬化兔晚期糖基化终末产物(AGEs)及其受体的影响。方法利用高脂饮食法诱导建立兔动脉粥样硬化模型。将新西兰白兔按随机数字表法随机分为正常对照组、动脉硬化组、吡哆胺小剂量组和吡哆胺大剂量组。正常对照组给予普通食物;动脉硬化组给予高脂饮食;吡哆胺小剂量组给予高脂饮食+吡哆胺100 mg/(kg·d);吡哆胺大剂量组给予高脂饮食+吡哆胺150 mg/(kg·d),共观察12周。实验12周末监测所有兔血糖、三酰甘油、总胆固醇、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C);应用酶联免疫吸附试验法检测血清AGEs水平;观察主动脉病理形态学改变;免疫组化方法检测主动脉AGEs受体(RAGE)含量,并进行各组间的比较。结果实验12周末,(1)与对照组比较,动脉硬化组兔三酰甘油、总胆固醇、LDL-C、HDL-C均明显增高(均P<0.01),血糖无明显变化(P>0.05);动脉硬化组的血清AGEs水平[(419±29)mg/L)]、动脉RAGE吸光度(0.88±0.14)及斑块面积[(67.4±5.8)%]均较对照组[分别为(141±11)mg/L、(0.19±0.04)及0%]明显增高(均P<0.01);(2)与动脉硬化组相比,吡哆胺小剂量组和吡哆胺大剂量组兔三酰甘油、总胆固醇、LDL-C、HDL-C、血糖均无明显变化(均P>0.05);吡哆胺小剂量组的血清AGEs水平[(278±22)mg/L]、动脉RAGE吸光度(0.43±0.06)及斑块面积[(43.2±2.4)%]和吡哆胺大剂量组的血清AGEs水平[(211±18)mg/L]、动脉RAGE吸光度(0.27±0.05)及斑块面积[(27.4±2.8)%]均较动脉硬化组明显减少(均P<0.01);(3)吡哆胺大剂量组的血清AGEs水平、动脉RAGE吸光度及斑块面积较吡哆胺小剂量组低(均P<0.01)。(4)主动脉病理形态学:显微镜下见动脉硬化组动脉内膜增厚,形成明显的粥样斑块;有大量的泡沫细胞聚集及较多的炎性细胞。吡哆胺小剂量组和吡哆胺大剂量组较动脉硬化组均有不同程度的减轻。结论吡哆胺能够抑制、减�
Objective To observe the effect of pyridoxamine on advanced glycosylation end-products （AGEs） and their receptors in arteriosclerotic rabbits. Methods A rabbit atherosclerotic model was induced and established by using a high fat diet. The New Zealand white rabbits were divided into a normal control group, an atherosclerosis group, a low-dose pyridoxamine group, and a high-dose pyridoxamine group according to the random number table method. The normal control group was given normal diet;the atherosclerosis group was given high-fat diet;the low-dose pyridoxamine group was given high-fat diet and pyridoxamine 100 rag/（ kg · d） ;and the high-dose pyridoxamine group was given high-fat diet and pyridoxamine 150 rag/ （kg· d）. All rabbits were observed for 12 weeks. Twelve weeks after the experiment,the blood glucose, triglyceride, total cholesterol, low-density lipoprotein cholesterol （ LDL-C ） , and high-density lipoprotein cholesterol （HDL-C） in all rabbits were monitored. The enzyme-linked immunosorbent assay was used to detect the serum advanced AGEs level. The changes of aortic pathological morphology were observed. Immunohistochemistry was used to detect the aortic receptor of AGEs （RAGE） content, and the comparison among the groups was conducted. Results Twelve weeks after the experiment： （ 1 ） compared with the normal control group, the triglyceride, total cholesterol, LDL-C, HDL-C were increased significantly （ P 〈 0.01） in the atherosclerosis group, but there was no significant change in glucose （ P 〉 0. 05 ）. Compared with the control group, the serum AGEs level （419 ± 29 mg/L） , artery RAGE absorbance （0.88 ±0.14）, and plaque area （67.4 ± 5.8% ） were increased significantly （ 141± 11 mg/L,0. 19 ± 0. 04 and 0%, respectively; all P 〈 0.01 ）. （2） Compared with the atherosclerosis group, the triglyceride, total cholesterol,LDL-C, HDL-C, and glucose did not have significantly change in the low-dose pyridoxamine group and the high-dose pyridox
出处
《中国脑血管病杂志》
CAS
CSCD
北大核心
2016年第11期588-592,600,共6页
Chinese Journal of Cerebrovascular Diseases
关键词
吡哆胺
动脉粥样硬化
晚期糖基化终末产物
晚期糖基化终末产物受体
Pyridoxamine
Arteriosclerosis
Advanced glycosylation end-products
Receptor for advanced glycation end-products
