摘要
微生物溶磷技术为充分利用我国丰富但难选的中低品位磷矿开辟了一条新的途径。旨在筛选出针对中低品位磷矿的高效溶磷菌,从湖北宜昌磷矿的酸性矿坑水中分离出一株兼性嗜酸异养菌A1。经菌株形态特征、生理生化指标和16S r DNA序列分析,鉴定属于Alicyclobacillus aeris。分析A1菌在4种不同培养基的生长及浸磷情况,结果显示,菌株A1在YSG培养基中生长及溶解中低品位磷矿的情况最佳,其中磷的浸出率高达77.22%,与无菌对照的化学浸出相比约提高了62%。根据原始磷矿和A1菌在YSG培养基中溶解的磷矿矿渣的物相分析及相应浸矿液的高效液相色谱检测,结果表明,A1菌发酵有机碳源代谢产生以草酸和柠檬酸为主的混合有机酸,磷矿石中的氟磷灰石、碳氟磷灰石经过这些有机酸的溶解,析出可溶性磷酸盐并同时生成石膏。
Bio-solubilization of phosphorous has pioneered a novel approach to make full use of rich but refractory middle-and low-gradephosphate ores in China,and this work aims to screen high-efficiency phosphorous-solubilizing bacteria for utilization of middle-and low-gradephosphate ores. A facultative heterotrophic strain A1 was isolated from the acid mine drainage of Yichang phosphate mines of Hubei province,and was identified as Alicyclobacillus aeris according to its morphological,physiological and biochemical characteristics,and 16 S r DNAsequence. For strain A1,YSG medium was optimal among four media regarding bacterial growth and its bio-solubilization of phosphorous fromphosphate ores;the fraction of phosphorous leached by strain A1 reached 77.22%,which increased about 62% compared to chemical leachingof abiotic control. According to X-ray diffraction（XRD）analyses of raw phosphate ores and bioleached residues in YSG medium as well ashigh performance liquid chromatography of the leachate,soluble phosphates were released by dissolving fluorapatite and carbonate-fluorapatitein phosphate ores via organic acid mixture（mainly oxilic acid and citric acid）generated during the fermentation of strain A1 with organiccarbon sources,and simultaneously gypsum was formed.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第11期224-234,共11页
Biotechnology Bulletin
基金
国家自然科学基金资助项目(21376184)
国家级创新创业训练计划项目(201410488011)
湖北省自然科学基金项目(2014CFB802)
湖北省科技厅基金项目(2009CDA006)
武汉科技大学青年科技骨干项目(2016xz014)
关键词
铜矿脂环酸芽孢杆菌
分离
鉴定
中低品位磷矿
生物浸出
Alicyclobacillus aeris
isolation
identification
middle-and low-grade rock phosphates
bioleaching
