摘要
为研究阿尔山地区落叶松根际固氮菌的多样性,试验采用平板分离法,结合16S r DNA序列比对,对落叶松根际土壤固氮菌组成类群及其多样性进行了分析。试验结果表明:自落叶松根际土壤共分离纯化细菌菌株28株,分属于10属18种,分别为假单胞菌属(Pseudomonas)、沙雷菌属(Serratia)、伯克氏菌属(Burkholderia)、根瘤菌属(Rhizobium)、叶杆菌属(Phyllobacterium)、类芽孢杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、红球菌属(Rhodococcus)、小杆菌属(Curtobacterium)、节杆菌属(Arthrobacter)。其中Pseudomonas、Phyllobacterium、Arthrobacter为优势菌属。研究结果阐明了阿尔山地区落叶松根际固氮菌的组成及其多样性。
In this paper plate isolation method,combined with 16S rDNA sequence analysis,were used to investigate the diversity and community structure of rhizosphere nitrogen-fixing bacteria isolated from Larix gmelinii in Arxan National Forest Park.A total of 28 isolates were obtained from L.gmelinii rhizosphere soil and their 16s rDNAs were sequenced and analyzed.The isolates were classified into 10 genera and 18 species.The genera isolated included Pseudomonas,Serratia,Burkholderia,Rhizobium,Phyllobacterium,Paenibacillus,Bacillus,Rhodococcus,Curtobacterium and Arthrobacter,with Pseu- domonas,Phyllobacterium,Arthrobacter are dominant groups.The results showed the community structure and diversity of nitrogen-fixing bacteria in the rhizosphere soil of L.gmelinii in Arxan National Forest Park.
作者
郭振华
陈立红
GUO Zhenhua;CHEN Lihong(Agricultural College,Inner Mongolia Agricultural University,Hohhot 010019,China)
出处
《北方农业学报》
2019年第2期73-78,共6页
Journal of Northern Agriculture
基金
国家自然科学基金项目(31260173)
