摘要
本研究以高氏1号培养基,采用平板涂布和划线分离培养法,从内蒙古巴彦淖尔市采集的盐碱地土壤中分离到一株放线菌(菌株编号BY104)。该菌的发酵液对引起马铃薯晚疫病的病原菌——致病疫霉的抑制率达100%;菌落的形态、生理生化特征和16S r DNA序列分析比较表明,菌株BY104属放线菌门的链霉菌属。该结果为利用该菌株开展马铃薯晚疫病的生物防治奠定了基础。
Using agar No. 1 medium,a actinomycete（ BY104） was isolated from a soil sample collected from Ba Yan Nao Er via gradient dilution plate method and streak plate method. BY104 showed a highter antimicrobial activity against Phytophthora infestans of Potato（ 100%）. According to its morphological,physiological and biochemical properties,combining the 16 S r RNA gene sequence,the strain was Streptomyces sp. The results may be potentially usefull for bio- control harmful Phytophthora infestans of Potato through this strain.
作者
杨永梅
张晓燕
甘霖
林睿
白薇
YANG Yongmei ZHANG Xiaoyan GAN Lin LIN Rui BAI Wei(College of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, China)
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2016年第3期72-76,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金项目(31560535)
关键词
放线菌
分离
鉴定
致病疫霉
Actinomycete
separation
identification
phytophthora infestans
