摘要
目的探讨去泛素酶基因(USP22)和叉头框M1基因(Fox M1)表达在结直肠癌发生、发展中的作用,以及与患者临床病理特征的关系。方法选取行手术切除的结直肠癌组织标本120例和正常结直肠黏膜组织标本32例;采用免疫组织化学En Vis ion二步法和We s te rn b lot检测结直肠癌组织及相应癌旁正常组织中USP22、Fox M1及Wnt/β-c a te nin通路相关蛋白(β-catenin、LEF/TCF、c-myc)的表达。结果结直肠癌组织中USP22、Fox M1的阳性表达率均明显高于癌旁正常组织(均P<0.05)。结直肠癌组织中USP22、Fox M1、β-c a te nin、LEF/TCF、c-myc蛋白的表达水平均明显高于癌旁正常组织(均P<0.05)。结直肠癌组织中USP22、Fox M1表达与肿瘤分化程度、淋巴结转移、肝脏转移及Duke's分期均有关(均P<0.05);且USP22/Fox M1共表达与肿瘤淋巴结转移、肝脏转移及Duke's分期亦均有关(均P<0.05)。结论结直肠癌组织中USP22、Fox M1的异常高表达可能参与了结直肠癌的发生、发展过程,且两者具有协同作用。
Objective To investigate the expression of Ubiquitin-specific protease 22 （USP22） and forkhead box M1（FoxM1） proteins in colorectal cancer and its clinicopathological significance. Methods The expression of USP22 and FoxM1 was detected by immunohistochemical EnVision method and Western blot in 120 specimens of colorectal cancer tissue and 32 specimens of normal colorectal mucosa tissue. Results The positive expression rate of USP22 and FoxM1 in colorectal cancer tissues were significantly higher than that in adjacent normal tissues （both P〈0.05）. The expression levels of USP22 and FoxM1 and Wnt/β-catenin proteins in colorectal carcinoma were significantly higher than those in adjacent normal tissue （all P〈0.05）.USP22 and FoxM1 expressions were significantly correlated with histological grade, lymph node metastasis, liver metastasis and Duke＇s stage （all P〈0.05）. The co-expression of USP22/FoxM1 was significantly correlated with lymph node metastasis, livermetastasis and Duke＇s stage in colorectal cancer patients （all P〈0.05）. Conclusion Results indicate that USP22 and FoxM1 proteins may be involved in the occurrence and the development process of colorectal cancer.
出处
《浙江医学》
CAS
2016年第21期1738-1741,共4页
Zhejiang Medical Journal
基金
嘉兴市第一医院"启明星"计划(2014-YA-02)
嘉兴市科技计划项目(2013AY21042-3)
浙江省自然科学基金项目(LY15H160067)
