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一种快速测定粗蛋白方法的初步研究 被引量:3

A preliminary study of a rapid method for crude protein determination
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摘要 为了获得一种不经过复杂消化过程即可测定棉粕和鱼粉中粗蛋白总含量的快速方法。实验以国标法作对照,利用过氧化氢代替国标法中的混合催化剂,不经过复杂消化即以酚酞作指示剂,通过蒸馏、滴定,快速测出样品中约60%的粗蛋白含量,经过换算系数1.715计算,得出样品中粗蛋白的总含量;对该方法稳定性进行试验。结果发现,快速法与国标法的结果一致,其稳定性良好。证明快速法是一种快速、可靠、稳定、高效的测量棉粕和鱼粉中粗蛋白总含量的方法。 To obtain a rapid method without the complicated catalysis process that can measure the to- tal crude protein content of the cottonse meal and fish meal. Compared with the GB method, using hy- drogen peroxide instead of the mixed catalyst, without complicated catalysis that using phenolphthalein as the indicator, by distillation, titration and quickly measure the crude protein content of about 60% of the samples, after conversion 1.715 coefficient calculation, the total crude protein content of the sample can be derived, And the stability test of the method. By comparison, rapid method consistent with the GB method, and good stability. Rapid method is a fast, reliable, stable and efficient measure- ment of the total amount of crude protein in cotton meal and fish meal.
出处 《饲料工业》 北大核心 2016年第19期50-53,共4页 Feed Industry
关键词 棉粕 鱼粉 粗蛋白 消化率 稳定性 cottonseed meal fish meal crude protein digestibility stability
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