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烟草蛋白酶体α2型亚单位的克隆和序列分析

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摘要 为研究烟草识别病毒并将其降解为蛋白酶体基因,以拟南芥的蛋白酶体α2型亚单位基因全长c DNA序列(Gen Bank登录号:AF043520.1)为信息探针,用电子克隆的方法从烟草栽培品种中克隆到1个蛋白酶体基因Nt PAB1,并对其进行序列分析。结果表明,Nt PAB1包含完整的开放读码框,编码219个氨基酸,含有1个保守域proteasome_alpha_type_2;通过同源比对和进化分析发现,Nt PAB1氨基酸序列与葡萄PAB1的序列一致性达到98%。以上结果表明,Nt PAB1是栽培烟草中未报道的编码蛋白酶体基因。
出处 《江苏农业科学》 北大核心 2016年第9期39-41,共3页 Jiangsu Agricultural Sciences
基金 贵州省科技厅科学技术基金(编号:黔科合J字[2014]2118号)
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