摘要
目的:观察人参皂苷Rb-2对肠道病毒71型(EV71)的抑制作用。方法:采用噻唑蓝(MTT)比色法检测人参皂苷Rb-2对人横纹肌肉瘤细胞(RD)的毒性作用;应用细胞病变法观察人参皂苷Rb-2浓度为6.25、12.5、25、50、75和100μg/m L时对EV71的抑制效应,通过检测病毒滴度观察药物对病毒的作用。结果:人参皂苷Rb-2(≤100μg/m L)对RD细胞无明显细胞毒性。人参皂苷Rb-2对EV71的抑制率呈浓度依赖性,50%抑制浓度(IC50)为20.8μg/m L。当人参皂苷Rb-2(≤100μg/m L)与EV71混合后处理RD细胞,未见药物对病毒的直接灭活作用。另外,人参皂苷Rb-2(75μg/m L)与EV71同时作用于RD细胞,或先将EV71加入到RD细胞,1 h后再加入人参皂苷,这两种方式对病毒滴度的影响无统计学意义。结论:人参皂苷Rb-2能有效抑制EV71复制。
Objective: To investigate the inhibitory effect of ginsenosides Rb-2 on enterovirus 71 (EV71). Methods: The cytotoxicity of ginsenosides Rb-2 on human embryonic rhabdomyosarcoma(RD) cells was detected by MTT method. The inhibitory effect of ginsenosides Rb-2 on EV71 was observed by cy- topathic effect method, and the influence of the drug on direct inactivating and absorbing effect was also studied. Results: When ginsenosides Rb-2 was at a concentration of ≤ 100 μg/mL,there was no significant cytotoxicity on RD cells. At 6.25, 12.5, 25, 50, 75 and 100 μg/mL, the inhibitory rates of ginsenosides Rb-2 against EV71 were (16.0 ±2.8)% ,(23.4 ±2.5)% ,(61.4 ±3.1)%, (77.9±2.9)% ,(98.3 ± 0.8 ) % and (98.6 ±1.0) %, respectively. The 50% inhibitory concentration was 20.8 μg/mL. Treated by the mixture of ginsenosides Rb-2 (≤〈1001xg/mL) and EV71 ,the direct inactivating effect on EV71 wasn't found in RD cells. When both ginsenosides Rb-2(75 μg/mL)and EV71 were added, or added at 1 hr post- infection, there was no statistical significance in viral titers. Conclusion: Ginsenosides Rb-2 can effectively inhibit EV71 replication.
出处
《江苏大学学报(医学版)》
CAS
2016年第3期215-218,共4页
Journal of Jiangsu University:Medicine Edition
基金
国家自然科学基金资助项目(81572052)
江苏省自然科学基金资助项目(BK20151178)