摘要
目的:了解普通蜂胶及纳米蜂胶对临床常见细菌的体外抑制效应。方法:K-B法测定不同浓度的普通蜂胶或纳米蜂胶对肺炎克雷伯菌、铜绿假单胞菌、伤寒沙门菌、金黄色葡萄球菌、表皮葡萄球菌、普通变形杆菌、大肠杆菌、肠球菌的抑菌作用。琼脂二倍稀释法测定普通蜂胶和纳米蜂胶对苯唑西林敏感金葡菌(MSSA)和耐药金葡菌(MRSA)的MIC。结果:普通蜂胶及纳米蜂胶对上述细菌的生长有一定的抑制作用,以对金黄色葡萄球菌的抑制作用最强,且随着浓度的升高抑制作用增加。除肠球菌和铜绿假单胞菌外,纳米蜂肢体外对上述细菌的抑制作用强于普通蜂胶。普通蜂胶对MSSA和MRSA的MIC_(50)和MIC_(90)均为128μg·ml^(-1)和256μg·ml^(-1);纳米蜂胶对MSSA和MRSA的MIC_(50)和MIC_(90)均为32μg·ml^(-1)和64μg·ml^(-1)。结论:蜂胶对多种细菌的生长有抑制作用,纳米蜂胶的抑菌作用强于普通蜂胶。但二者对金葡菌的抑菌作用均明显低于苯唑西林和万古霉素。
Objective：To investigate the anti-bacterium effects of Chinese propolis and nano-propolis on common pathogens in vitro. Method： The susceptibility of Staphylococcus aureus, S. epidermis, Enterocuecus , Klesiella pneumoniae , Pseudomonas aeruginosa, Sahnonella typhii, Proteus vulgaris and Escherichia coli to Chinese propolis and nano-propolis was detected by K-B method. The minimal inhibitory concentrations（MIC） of Chinese propolis and nano-propolis to metieillin-sensitive S. aureus （MSSA） and meticillinresistance S. aureus （MRSA） were detected by agar double dilution method. Result： The Chinese propolis could inhibit the growth of all bacteria, but it was more effective to Gram positive bacteria than Gram negative bacteria. The nano-propolis showed more effective antimicrohial activity than Chinese propolis. MIC50 and MIC90 of Chinese propolis to MSSA and MRSA were 128μg·ml^-1 and 256μg·ml^-1,Fespectively; MIC50 and MIC90 of nano-propolis to MSSA and MRSA were 321μg·ml^-1 and 64μg·ml^-1,Fespectively. Conclusion：Both Chinese propolis and nano-propolis can inhibit bacteria effectively, nano-propolis shows more effective inhibitory activity. However their inhibitory effect on S. aureus was obviously weaker than oxacillin and vancomycin.
出处
《中国药师》
CAS
2008年第10期1167-1169,共3页
China Pharmacist
关键词
蜂胶
纳米化
细菌
抗菌活性
Propolis
Nano-propolis, Bacterium
Antimicrobial activity
