摘要
目的:检测转移抑制因子1(metastasis suppressor 1,MTSS1)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在结直肠癌中的表达变化及临床意义。方法:在含有240例结直肠癌标本的组织芯片上,利用免疫组织化学法及qRT-PCR法检测MTSS1和VEGF在结直肠癌、癌旁组织中的表达,并分析与结直肠癌临床参数的关系。结果:结直肠癌组织中MTSS1阴性表达率和VEGF阳性表达率明显高于癌旁组织(P<0.05)。MTSS1阴性表达与淋巴结转移、Duke's分期相关(P<0.05);VEGF阳性表达与患者年龄(≥60岁)、肿瘤分化程度(低分化)相关(P<0.05)。结论:结直肠癌中MTSS1低表达和VEGF高表达可能与结直肠癌的发生发展及转移有关。
Objective： To investigate expression of metastasis suppressor 1 （MTSS1）and vascular endo- thelial growth factor（VEGF） and their prognostic significance in colon cancer. Methods： We constructed tissue microarray blocks containing colon cancer specimens from 240 patients. Expression of MTSS1 and VEGF in the specimens were analyzed using immunohistochemistry （IHC） technique and qRT-PCR. And their correlation with clinical parameters were analysed. Results： The negative expression rate of MTSS1 and the positive expression rate of VEGF was much higher in colon cancer than in adjacent tissue （P 〈 0.05）. Negative MTSS1 expression was significantly associated with presence of lymph node metastasis and advanced Duke＇s stages（P 〈 0.05）. The over-expression of VEGF had a significant relationship with age （≥60 years） and poorly differentiation （P 〈 0.05）. Conclusion： Decreased expression of MTSS1 and overexpression of VEGF may be related to the the development and metastasis of colon cancer.
作者
杨洁
韩玮
王永强
曹方
丁厚中
YANG Jie HAN Wei WANG Yong-qiang CAO Fang DING Hou-zhong(Department of Anorectal Surgery Department of General Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan Jiangsu 215300, China)
出处
《江苏大学学报（医学版）》
CAS
2015年第6期520-523,共4页
Journal of Jiangsu University：Medicine Edition
关键词
结直肠癌
转移抑制因子1
血管内皮生长因子
colon cancer
metastasis suppressor 1
vascular endothelial growth factor
