摘要
目的探讨结直肠癌组织中DNA结合分化抑制蛋白1(Id-1)和基质金属蛋白酶9(MMP-9)的表达及其与肿瘤微血管生成、临床病理指标的相关性。方法应用免疫组织化学染色法检测50例结直肠癌组织和50例癌旁正常组织中Id-1和MMP-9的表达,以及CD34标记的微血管密度(MVD)情况。结果 Id-1和MMP-9在结直肠癌组织中阳性表达为72.00%(36/50)、78.00%(39/50),显著高于癌旁正常组织的24.00%(12/50)、28.00%(14/50)(P=0.000);结直肠癌组织中MVD值为17.22±2.08,明显高于癌旁正常组织5.36±2.17(P=0.000);Id-1、MMP-9和MVD均与肿瘤浆膜浸润、TNM分期、癌胚抗原(+)、脉管浸润、淋巴结转移及肝转移相关(P均<0.05),而与年龄、性别、肿瘤大小、分化程度无关(P均>0.05);癌组织中Id-1和MMP-9阳性表达组MVD值显著高于阴性组(P均=0.000);结直肠癌组织中Id-1与MMP-9呈明显正相关性(r=0.429,P=0.000);生存分析显示:Id-1与MMP-9表达与结直肠癌患者预后关系密切,二者高表达提示预后不良。结论 Id-1、MMP-9高表达与结直肠癌的发生演进高度相关,并与MVD呈正相关,二者可能共同参与了肿瘤微血管的生成、肿瘤的浸润及血行转移。
Objective To explore the expressions of inhibitors of DNA binding-1（ Id-1） and matrixmetalloproteinase-9（ MMP-9） in colorectal carcinoma tissues and its correlation with microvessel density（ MVD）.Methods The expressions of Id-1 and MMP-9 as well as CD34-labelled MVD in colorectal adenocarcinoma tissues（ n = 50） and normal adjacent tissues（ n = 50） were examined by immunohistochemistry. Results The positive expressions of Id-1 and MMP-9 were seen in 72. 00%（36 /50） and 78. 00%（39 /50） of colorectal adenocarcinoma tissues,which were significantly higher than those [24. 00%（12 /50） and 28. 00%（14 /50）]in normal adjacent tissues（ P = 0. 000）. The MVD value（ 17. 22 ± 2. 08） in colorectal adenocarcinoma tissues was significantly higher than that（5. 36 ± 2. 17） in normal adjacent tissues（ P = 0. 000）. The expressions of Id-1 and MMP-9 and MVD were significantly correlated with serosa invasion,TNM stage,carcinoembryonic antigen（ ＋）,lymph node metastasis,vascular invasion,and liver metastasis（ all P〈0.05） but not with the patient＇s age,gender,tumor size,and differentiation degree（ all P〉0.05）. The MVD value with Id-1 and MMP-9 positive expression were significantly higher than those with Id-1 and MMP-9 negative expression（ all P = 0. 000）. The expression of Id-1 in colorectal adenocarcinoma tissues showed significantly positive correlation with that of MMP-9（ r = 0. 429,P = 0. 000）. Cox multivariate analysis showed that Id-1 and MMP-9 expressions were independent prognostic factors for colorectal carcinoma. Conclusions The high expressions of Id-1and MMP-9 have high correlations with the development and progression of colorectal adenocarcinoma and have positive correlation with MVD. Both of them may be involved in the microvascular generation and the invasion and hematogenous metastasis of colorectal carcinoma.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2016年第6期696-701,共6页
Acta Academiae Medicinae Sinicae
基金
河北省卫生计生委医学科学研究重点课题(20150058)
河北省科技支撑计划项目(152777237)
河北省张家口市科技局指令性计划(1311055D)~~
