摘要
目的探讨miR-96和转移抑制因子1(MTSS1)在前列腺癌(PCa)组织中的表达及其临床意义。方法收集PCa患者标本89例,癌旁组织作为正常对照,分别用原位杂交及免疫组化检测PCa组织及癌旁组织中miR-96和MTSS1的表达水平,并对二者进行相关性分析。结果 89例PCa组织中miR-96阳性表达率为87.64%,显著高于癌旁组织(P<0.01);而MTSS1蛋白阳性表达率为16.85%,显著低于癌旁组织(P<0.01);miR-96的表达随着Gleason评分和临床分期演进而增加(P<0.05),有骨转移的miR-96的表达高于无骨转移组(P<0.05);MTSS1的表达随着Gleason评分和临床分期演进而下调,且有骨转移的MTSS1的表达显著低于无骨转移组(P<0.01);miR-96表达与MTSS1的表达呈显著负相关(P<0.01)。结论 miR-96与MTSS1可能是PCa骨转移的重要生物学标志。
Objective To investigate the expression of the miR-96 and metastasis suppressor 1 （ MTSS1 ） in prostate cancer and their clinical significances. Methods Compaired with adjacent tissue, hybridization in situ and immu- nohistochemistry were used to detect the miR-96 and MTSS1 expression in collected prostate cancer tissue speci- mens from 89 cases patient. Results The positive expression rate of the miR- 96 and MTSS1 were 87.64% , 16. 85% in respectivly prostate cancer （P 〈 0. 01 ）. The up-regulation of miR-96 expression was associated with Gleason score and advanced clinical stage （ P 〈 0.05 ） , and the expression of miR-96 was higher with bone metasta- sis than that without bone metastasis in prostate cancer（P 〈 0. 05）. But the down-regulation of MTSS1 expression was associated with Gleason score and advanced clinical stage（P 〈 0.05） , the expression of MTSS1 was lower with bone metastasis than without bone metastasis in prostate cancer（P 〈0.01 ）. The expression of miR-96 had a close negative correlation to that of MTSS1 in prostate cancer （P 〈 0. 1 ）. Conclusions miR-96 and MTSS1 may be im- portant biological markers of bone metastasis in prostate cancer.
出处
《基础医学与临床》
CSCD
2016年第7期1010-1013,共4页
Basic and Clinical Medicine
