摘要
对不同产地、不同采收期及不同部位叶下珠药材柯里拉京含量测定进行比较.分析采用Hypersil ODS2（250 mm×4.60 mm,5 um）色谱柱,以乙腈（A）-0.1%磷酸水溶液（B）二元系统梯度洗脱,柱温25℃,流量0.8 m L/min,检测波长280 nm.柯里拉京在0.458~4.580 mg/g（r=0.995 9）呈良好的线性关系,加样回收率（n=5）为97.98%.结果表明：不同产地叶下珠药材柯里拉京的含量有较大差异,广东中山的含量最高达4.224 mg/g;不同采收期叶下珠药材柯里拉京含量在10月5日达到最高,随后下降,建议叶下珠药材的采收期为每年的10月上旬;不同部位叶下珠药材柯里拉京含量差异较大,根含量很低,建议叶下珠药材只采收地上部分.本实验建立HPLC法测定叶下珠药材柯里拉京含量,该方法简便、准确、重现性好,可为叶下珠药材质量评价及质量控制提供依据.
To compare the content in corilagin of Phyllanthus urinaria L.from different habi- tats,different harvest time ,different parts.The analysis was carried out on a Hypersil ODS2 column （250 mm ×4.60mm, 5μm）, mobile phase was aeetonitrile （ A ） - 0. 1% phosphoric acid aqueous（B） with gradient elution, column temperature was maintained at 25℃, flow rate was 0.8 mL/min and detection wavelength was set at 280 nm.Corilagin showed good lin- ear relationships within the ranges of 0.458-4.580 mg/g（r = 0.995 9） ,whose average recov- eries（n=5） were 97.98%.The results was as follows：the content of corilagin in Phyllanthus urinaria L.varied greatly with different habitats,the highest is from Zhongshan City, Guang- dong Province at 4.224 mg/g. The content of corilagin increased with the growth period, reached the highest in October 5th.It was suggested the best harvest time is early October each year.The content of eorilagin from root was very low, therefore, suggested only collecting the aerial parts.This method is simple ,accurate with good reproducibility,which can be used for the quality control of Phyllanthus urinaria L.
出处
《南华大学学报（自然科学版）》
2016年第3期98-102,共5页
Journal of University of South China：Science and Technology
基金
湖南省教育厅青年基金资助项目(04B018)
衡阳市科技局科技计划基金资助项目(2007KJ014)
关键词
高效液相色谱法
叶下珠
柯里拉京
比较研究
high performance liquid chromatography
Phyllanthus urinaria L.
corilagin
comparative study
