摘要
目的:优选叶下珠中以柯里拉京为主的溶栓成分的提取工艺。方法:以溶剂量、提取时间和提取次数为考察因素,以浸出物和柯里拉京含量的综合评分为指标,采用正交试验优选提取工艺;考察离心法、酸碱法、乙醇沉淀法的除杂质效果。结果:最佳提取工艺为以8倍量30%乙醇为溶剂,回流提取3次,每次2h;90%的乙醇除杂质效果最佳。结论:该工艺简便、稳定,提取物有效成分含量高,可为进一步的开发提供理论依据。
OBJECTIVE： To optimize the extraction technology of thrombolytic components predominantly as corilagin from Phyllanthus urinaria. METHODS： The extraction technology was optimized by orthogonal experiment with solvent amount, extracting time and extracting times as factors, and with the comprehensive score of the yield of extract and corilagin content as indexe. The impurity removing efficacy by centrifugation, acid/ base method or ethanol precipitation was evaluated. RESULTS： The optimal extraction technology was as follows： using 8-fold volume of 30% ethanol as solvent for reflux extraction of 3 times （2 hours/time） . The best impurity- removing efficacy was achieved when 90% ethanol was used for precipitation. CONCLUSION： The extraction technology is simple and stable with high yield of active components, and it provides theoretic basis for the industrial production.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第33期2584-2586,共3页
China Pharmacy
基金
广东省中医院中医药科学技术研究专项"名医工作室研究"项目(2007E199)
关键词
叶下珠
柯里拉京
提取
纯化
Phyllanthus urinaria
Corilagin
Extraction
Purification
