摘要
本文主要利用高效液相色谱法对玄参芦头片和根片中5种成分(哈巴苷、麦角甾苷、安格洛苷C、哈巴俄苷、肉桂酸)进行含量测定,以探讨玄参芦头片对玄参药材整体质量的影响。首先,采用高效液相色谱法,在210、280、330 nm下对样品进行检测。其次,采用紫外分光光度法,在517 nm下对样品提取液的DPPH自由基清除率进行检测。玄参中所测定的5种成分均达到基线分离,其芦头片与根片提取液的DPPH自由基清除率无显著差异。各批玄参饮片中普遍存在芦头片,实验结果表明玄参芦头片和根片中的哈巴苷和哈巴俄苷总含量差异较小,因此芦头片对玄参药材整体质量基本没有影响。
A high performance liquid chromatograph（ HPLC） method was established for the simultaneous determination of five components（ Harpagide,Verbascoside,Angoroside-C,Harpagoside,Cinnamic acid） in Scrophulariae Rhizome and Scrophulariae Radix,in order to investigate the impact of Scrophulariae Rhizome on the overall quality of Scrophulariae Radix. The determination was performed on a Waters 2695 HPLC with a 2998 DAD detector under 210,280 nm and330 nm. In addition,the DPPH free radical scavenging rates of Scrophulariae Radix were determined at 517 nm by Ultraviolet Spectrophotometry. Consequently,the five components reached the baseline separation and there were no significant differences in the DPPH free radical scavenging rates between the extracts of Scrophulariae Rhizome and Scrophulariae Radix. Each batch of Scrophulariae Radix generally contained Scrophulariae Rhizome,and the total contents of Harpagide and Harpagoside in Scrophulariae Rhizome was similar to those in Scrophulariae Radix. Therefore,the existence of Scrophulariae Rhizome had little impact on the overall quality of Scrophulariae Radix.
作者
许一鸣
吴啟南
乐巍
蒋征
桑梦如
吴达维
XU Yi-ming WU Qi-nan YUE Wei JIANG Zheng SANG Meng-ru WU Da-wei(College ofpharmacy,Nanjing University of Chinese Collaborative Innovation Center of Chinese Medicinal Resources Industriahzation Nattonal and Local Collaborative Engineering Centerof Chinese Medicinal Resources lndustrialization and Formulae Innovative Medicine ,Nanjing 210023, China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第9期1414-1421,共8页
Natural Product Research and Development
基金
江苏省高校优势学科建设工程资助项目
中医药行业科研专项(2015468002-3)
