摘要
目的探讨职业接触六价铬对工人线粒体DNA中MT-TF基因甲基化水平的影响。方法采用水浴酸消化一电感耦合等离子质谱法检测人群血铬水平,采用胞质阻滞双核微核法检测人群微核率,采用Mass ARRAY分子量阵列技术检测MT-TF基因甲基化水平。结果 57名手工电镀铬工人为暴露组人群的血铬水平为(10.21±1.35)mg/L,明显高于88名无职业接触史工人为对照组的(3.57±0.43)mg/L,差异有统计学意义(P<0.05);暴露组人群双核微核率为(3.23±0.35)‰,明显高于对照人群(2.22±0.24)‰,差异具有统计学意义(P<0.05);暴露组人群中MT-TF基因甲基化水平为(4.41±0.17)%,明显低于对照组人群甲基化水平(5.19±0.24)%,差异具有统计学意义(P<0.05);相关分析结果显示,被调查人群血铬水平与MT-TF基因甲基化水平有相关关系(r=-0.169 2,P=0.041 9),随着血铬水平增加,MT-TF基因甲基化水平有降低趋势。结论职业接触六价铬可引起机体遗传损伤和表观遗传损伤,MT-TF基因DNA甲基化水平降低是潜在的职业铬暴露的生物标志。
Objective To determine the effect of occupational exposure to chromate（Cr）on the DNA methylation levelof MT-TF gene in Cr-exposed workers. Methods Serum concentration of chromium was detected by inductively coupledplasma mass spectrometry（ICP-MS）,and the micronucleus rate was measured by cytokinesis-block micronucleus（CBMN）test. Sequenom Mass ARRAY platform was applied to inspect the DNA methylation level of mitochondrial and nuclear DNA.Results When compared with the control group,the Cr- exposed group had significantly higher serum chromium ionconcentration [（10.21±1.35）vs（3.57±0.43）mg/L,P〈0.05）],higher micronucleus rate [（3.23±0.35）‰ vs（2.22±0.24）‰,P〈0.05],but lower DNA methylation level of MT- TF gene [（4.41 ± 0.17）% vs（5.19 ± 0.24）%,P〈0.05]. Correlation analysisshowed that the serum Cr ion concentration was correlated with the methylation level of MT-TF gene（r=-0.169 2,P=0.041 9）,with the higher the Cr ion concentration the lower methylation level. Conclusion Occupational exposure to chromate inducesboth genetic and epigenetic damage. The decreased methylation level of MT- TF is a potential biomarker of occupationalexposure to Cr.
作者
杨淋清
吴德生
黄海燕
丁鸿
杨学琴
张慧敏
崔栋
刘建军
庄志雄
YANG Lin-qing WU De-sheng HUANG Hai-yan DING Hong YANG Xue-qin ZHANG Hui-min CUI Dong LIU Jian-jun ZHUANG Zhi-xiong(Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, China)
出处
《中国热带医学》
CAS
2016年第9期851-854,共4页
China Tropical Medicine
基金
国家自然科学基金(No.81273127
81172710
81473014)
