摘要
[目的]比较猴头菌子实体、菌丝体粗提物理化性质,以及2组粗多糖对胃粘膜上皮细胞氧化模型保护能力的差异。[方法]采用水提醇沉法提取粗多糖,用苯酚-浓硫酸法测定葡萄糖含量、间羟基联苯法测糖醛酸含量、BCA法测量蛋白质含量。每组分别加入一定浓度的含粗多糖的培养基,分别培养12、24、48 h后加入含过氧化氢培养基。用MTT法测细胞活度。[结果]猴头菌菌丝体、子实体粗多糖中葡萄糖含量分别为49.5%、44.5%,糖醛酸含量分别为22.01%、29.23%,蛋白质含量分别为27.69%、23.54%。菌丝体组12、24、48h抑制率分别为75.1%、68.1%、16.9%;子实体组分别为70.2%、61.8%、16.7%。[结论]猴头菌菌丝体与子实体粗多糖中糖醛酸、葡萄糖、蛋白质含量相近。子实体粗多糖比菌丝体粗多糖抗氧化活性短时间内略强,长时间无差异。
[Objective] To compare the physicochemical properties of crude polysaccharides compare of Hercium erinaceus mycelium and fruit body, and to compare the differences of the effects of two crude extracts on oxidation protection ability of gastric mucosa epithelial cell.[ Method] Crude crude polysaccharides were extracted by water-extraction and alcohol-precipitation method.Glucose content was detected by phenol-sulphuric acid method; uronic acid content was detected by m-hydroxydiphenyl method; and protein content was detected by BCA method. Culture medium containing certain content of polysaccharides compare was added into each group.After cultivated for 12, 24 and 48 h, cul-ture medium containing hydrogen peroxide was added.Viability of cells was detected by MTT.[ Result] Glucose contents in mycelium and fruit body were 49.5% and 44.5%, respectively.The uronic acid contents were 22.01% and 29.23%; protein contents were 27.69% and 23.54%.The inhibitory rates of mycelium group at 12, 24 and 48 h were 75.1%, 68.1% and16 .9%, respectively;those of fruit body were 70.2%, 61.8% and 16.7%, respectively.[Conclusion]In the crude crude polysaccharides of mycelium and fruit body of Hecr ium erina-ceus, contents of uronic acid, glucose and protein are close.Crude polysaccharides of fruit body have stronger antioxidant activity than extracts of mycelium in short term, but there were no differences in long term.
出处
《安徽农业科学》
CAS
2016年第24期123-124,154,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30873370)
关键词
猴头菌菌丝体
猴头菌子实体
理化性质
胃粘膜细胞
抗氧化
Hericium Erinaceus mycelium
Hercium erinaceus fruit body
Physicochemical properties
Gastric mucosal cell
Antioxidant
