摘要
目的区分D基因型重组类型,了解青海地区乙型肝炎病毒(hepatitis B virus,HBV)感染者C/D基因型重组情况.方法收集217份青海地区慢性乙型肝炎感染者样品,PCR扩增535-1460 nt和1779-2400 nt2个片段的乙型肝炎病毒基因序列,分2区(593-799 nt、799-1450 nt和1799-2400 nt)构建进化树,确定青海地区HBV基因重组类型.利用INNO-Li PA HBV分型试剂检测青海地区的C/D基因型重组样品,计算符合率.结果青海地区利用HBV不同区域进化树分型,结果显示各基因型比例为CD1(61.9%)、CD2(8.4%)、C(27.0%)和B(2.8%).基因型分布在藏族和汉族乙型肝炎感染者中的差异具有显著的统计学意义(?2=17.9,P<0.01),藏族乙型肝炎感染者中C/D基因型重组和C基因型比例为9 1.5%和8.5%;在汉族中的比例为68.5%和32.3%.C/D基因型重组和C基因型HBV e抗原阴阳性的差异(?2=0.28,P>0.05)以及病毒载量的差异(t=1.125,P>0.05)均没有统计学意义.C/D重组样品INNO-Li PA分型试剂检测结果为D基因型的理论符合率为87.8%.结论青海地区乙型肝炎感染者以CD1(10-799 nt)基因型重组以主,对其临床意义应进一步的研究分析.该地区的HBV分型检测应注意基因重组对分型试剂的影响.
AIM To distinguish two forms of hepatitis B virus（HBV） C/ D recombinant and investigate the distribution of HBV C/ D recombinant genotypes in Qinghai Province.METHODS A total of 217 chronic HBV infected serum samples were collected from Qinghai Province.Two fragments of HBV,535-1460 nt and1779-2400 nt,were amplified and sequenced.The two forms of C/ D recombinant were confirmed by the phylogenetic tree constructed based on593-799 nt,799-1450 nt and 1799-2400 nt.The samples of C/ D recombinant were determined by INNO-LiPA HBV genotyping assay.RESULTS The distribution of HBV genotypes in Qinghai Province detected by the phylogenetic tree based on different fragments was as follows：CD1（61.9%）;CD2（8.4%）;C（27.0%） and B（2.8%）.Ethnically,the C/ D recombinant had a higher prevalence in Tibetan patients（91.5%） than in Han populations（62.6%）.The distribution of HBV genotypes in Tibetan patients was significantly different from that in Han populations（χ~2 = 17.9,P 0.01）.Clinically,there was no significant difference in viral load between Tibetan and Han populations（F= 0.68,P 0.05）,and between males and females.There was no significant difference in HBeAg positive/ negative ratios or HBV viral load between the C/ D recombinant genotype and genotype C（χ~2 = 0.28,P 0.05;t = 1.125,P 0.05）.The majority（87.8%） of samples of C/ D recombinant were reported to be genotype D by INNO-LiPA HBV genotyping assay.CONCLUSION The CDl（10-799 nt） recombinant of HBV is the dominant genotype in Qinghai Province.The clinical outcomes of different C/ D recombinant genotypes should be further studied,and the results of INNO-LiPA HBV genotyping assay should be reinterpreted in Qinghai Province.
出处
《世界华人消化杂志》
CAS
2016年第26期3779-3789,共11页
World Chinese Journal of Digestology
基金
十二五传染病重大专项课题基金资助项目
No.2012ZX10004701~~
关键词
乙型肝炎病毒
基因型
重组
Hepatitis B virus
Genotype
Recombination
