摘要
目的:探析血清筛查阴性相关核酸阳性献血者乙型肝炎相关病毒感染的特征。方法:选取2016年1月-2018年1月献血者标本4500份,使用两种不同酶联免疫吸附试剂检测乙肝表面抗原(HBsAg)。测定酶免阴性、单试阳性标本核酸,并对乙肝阴性、核酸阴性者随访分析。结果:使用核酸血液标本2430份,HBsAg酶免检测阴性2410份,单试标本阳性20份;筛查乙型肝炎病毒呈阳性43份,确定为阳性34份,阳性检出率和阳性确认率分别为17.69‰、13.99‰,HBV相关酶联免疫吸附实验(ELISA)漏检率为10.28‰。结论:对献血者进行HBV的血液筛查,采用核酸检测能够有效提高灵敏性,并降低窗口期,从而提高安全性。
Objective:To investigate the characteristics of hepatitis B virus infection in positive blood donors with serological screening negative nucleic acid test.Methods:From January 2016 to January 2018,4500 samples of blood donors were selected,HBsAg were detected by using two different enzyme-linked immunosorbent assays.The negative of enzyme-linked immunosorbent assays and single-test positive samples of nucleic acid were detected,hepatitis B negative and nucleic acid negative were follow-up analyzed.Results:2430 samples of nucleic acid blood were used,2410 samples were negative for HBsAg enzyme-linked immunosorbent assay and 20 samples were positive for single test;Screening for 43 hepatitis B viruses was positive and 34 were positive.The positive detection rate and positive confirmation rate were 17.69‰and 13.99‰,respectively,the missed detection rate of HBV-related ELISA was 10.28‰.Conclusion:Blood screening of hepatitis B virus by blood donors and the use of nucleic acid detection can effectively improve sensitivity and reduce the window period,thereby improving safety.
作者
王晓梅
段荣
郭滔
Wang Xiaomei;Duan Rong;Guo Tao(Department of Clinical Laboratory,the Center Blood Station of Hengyang City,Hunan Hengyang 421000)
出处
《中国社区医师》
2020年第11期135-136,共2页
Chinese Community Doctors
关键词
血清学筛查
核酸检测
献血者
乙型肝炎病毒
Serological screening
Nucleic acid detection
Blood donors
Hepatitis B virus
