摘要
目的建立一种准确、高效的HPLC同时检测多类中成药及保健品中非法添加的16种糖皮质激素。方法采用Agilent Extend-C_(18)(250 mm×4.6 mm,5μm)为色谱柱;乙腈-0.1 mol·L^(-1)乙酸铵溶液为流动相,梯度洗脱,检测波长为240 nm。结果 16种糖皮质激素在0.2~25μg·L^(-1)内线性良好;各成分最低定量限浓度均<0.05 ng·m L^(-1);回收率,除了贴膏剂(57.2%~83.8%),其余剂型均获得满意结果(≥78.5%)。结论本方法简单高效,结果准确可靠,通用性强,可作为同时检测中成药及保健品中非法添加的16种常用糖皮质激素的有效方法。
OBJECTIVE To establish an accurate, universal and efficient HPLC method for simultaneous determitation of 16 glucocorticoids illegally added in Chinese patent medicine and health products. METHODS HPLC method was performed on an Agilent Extend-C18（250 mm×4.6 mm, 5 μm） column with the mobile phase consisted of 0.1 mol·L^-1 ammonium acetate solution-acetonitrile in a gradient elution mode. The detector wavelength was set at 240 nm. RESULTS The linear range of 16 glucocorticoids were 0.2-25 μg·mL^-1, which showed a good correlation. The recoveries were good except plaster（≥78.5%）, except the emplas trum with the recovery rate of 57.2%-83.3%, and the detection limits were all 〈0.05 ng·mL^-1. CONCLUSION The method is simple and efficient, accurate and universal. It can be used as an efficient method for determination of 16 commonly used glucocorticoids which are illegally added in Chinese patent medicine and health products.
出处
《中国现代应用药学》
CAS
CSCD
2016年第9期1178-1182,共5页
Chinese Journal of Modern Applied Pharmacy
