摘要
本研究利用ISSR—PCR分子标记技术分析研究了重庆市南川区五个地方人工种植玄参的遗传多样性，利用7条UBC引物共扩增出了61条DNA片段，其中多态性片段为48条．片段长度约为250～2000bp，每条引物扩增出的DNA片段数为4～14条．同时采用NTSYS_pc2．1软件计算出五个区域之间和区域内部子区域的玄参的Nei’S遗传距离，并用UPGMA法分别构建了系统树．结果表明五个地方人工种植玄参存在一定的遗传多态性，但其地方之间差异并不明显，其遗传多样性主要在同一地方内的子区域间被观察到．
Genetic diversity of trueborn Scrophularia ningpoensis Hemsl in five areas of Nanchuan dis- trict, Chongqing was preliminary analyzed using inter-simple sequence repeat （ISSR） marker. Seven UBC primers were used in this research, and a total number of 61 DNA fragments were obtained, with 48 were polymorphic in total. The range of the numbers of DNA fragments amplified by each primer was 4 to 14. The Nei＇s genetic distances of the five areas and their subareas were calculated using NTSYS- pc2.1 software and the dendrogram were constructed through UPGMA method. The results revealed that the genetic diversity of Scrophularia ningpoensis Hemsl cultivated separately in those five areas could be observed, but it was not obvious among the five big areas but their subareas.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第5期1119-1124,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家工信部中药材生产项目(2013-37)
关键词
玄参
种植中药材
ISSR
遗传多样性
Scrophularia ningpoensis Hemsl.
Cultural Chinese medicine herb.
Inter-simple sequencerepeat
Genetic diversity
