摘要
样品在酸性条件下通过加入邻硝基苯甲醛实现衍生化,乙酸乙酯萃取,浓缩,以乙腈∶水（20∶80）定容和正己烷除脂后,采用液相色谱-串联质谱法检测黄鱼鲞中硝基呋喃代谢物残留量。该方法中4种硝基呋喃代谢物在1~50μg/L浓度范围内呈现良好的线性关系,相关系数均大于0.9971,定量限均为0.5μg/kg。4个添加水平（1μg/kg、2μg/kg、5μg/kg、10μg/kg）的平均回收率在89.3%~106%之间,相对偏差均小于7.03%。结果表明该方法满足黄鱼鲞中硝基呋喃代谢物的检测要求。
The samples were derived with 2-nitrobenzaldehyde in hydrochloric acid solution. After extracted with ethyl acetate and concentrated with rotary evaporator, the analytes were dissolved with acetonitrile solution （CH3 CN： H2O= 20： 80）, defatted with n-hexane and determined by LC-MS/MS. The results showed that the calibration curves for the analytes exhibited good linearity over the concentration range from 1 to 50 μg/L with the LOQ of 0.5μg/kg. Average recoveries for the spiked samples at4 levels （≥g/kg, 2μg/kg, 5μg/kg, 10μg/kg） ranged from 89.3% to 106%, with a RSD less than 7.03%. The method is suitable for the determination of nitrofurans metabolites in dried salted yellow croakers.
出处
《分析仪器》
CAS
2016年第5期14-18,共5页
Analytical Instrumentation
基金
福建省质量技术监督局科技项目(FJQI2013112)
