摘要
建立液相色谱-质谱/质谱法测定饲料中四环素类（四环素、土霉素、金霉素、强力霉素）药物残留的方法,旨在提高检测效率和检测限度。实验中,采用Na2EDTA-Mcllvainc缓冲溶液提取样品,经Phenomenex Strata-X固相萃取柱净化,进行液相色谱-质谱/质谱法分析。结果表明：四环素类标准品3个添加浓度20、50和100μg/kg的平均回收率为93%～103%,相对标准偏差小于15%,四环素类药物的检测定量限（LOQ）：20μg/kg,检出限：5μg/kg。标定曲线的线性范围：10～200μg/L,R〉0.998。此方法适用于饲料中四环素类药物残留检测。
A high performance liquid-MS/MS detection method was established for the simultaneous determination of multi- ple trtracyelines, TCs （TC, OTC,CTC,DOXY）residues in feedstuff. TCs in feedstuff sample was extracted with Na2 EDTA- Mellvaine buffer solution, after centrifugation, cleaned with a Waters Oasis HLB SPE cartridge, and then detected by HPLC- MS/MS. Results showed that the average recoveries of the tetracycline at the three concentrations of （20, 50 and 100 μg/kg）, were from 93 % to 103 % with RSD 〈15 %. The limit of quantity（LOQ） to TCs：20 μg/kg. The calibration curve was linear in the range of 10～ 200 μg/L and R〉0. 998. The method was suitable for the detection of TCs residues in feedstuff.
出处
《粮食与饲料工业》
CAS
2016年第9期61-63,共3页
Cereal & Feed Industry
基金
湛江市财政资金科技项目(2012E0202)
关键词
饲料
四环素类
固相萃取
液质联用
检测
feedstuff
multiple trtracyclines
SPE
HPLC-MS/MS
determination
