摘要
乳白蛋白与油酸形成的复合物具有癌细胞生长抑制作用（Human alpha-lactalbumin made lethal to tumor cells,HAMLET）。本文制备了卵白蛋白（Ovalbumin,OVA）和油酸（Oleic acid,OA）的复合物（OVA-OA）,通过检测细胞生长抑制、细胞膜通透性、细胞生长周期和凋亡的变化,分析了OVA-OA对人结肠癌细胞（Caco-2）的生长抑制作用以及其中OA对生长抑制作用的贡献。结果表明,当OVA-OA中OA的浓度达到182μmol/L时,OVA-OA对Caco-2细胞有显著（p〈0.05）生长抑制作用,273μmol/L时生长抑制作用增强,且随着作用时间的延长（1～3 h）而增强。在182μmol/L时,OA对Caco-2细胞也有生长抑制作用,其剂量效应关系与OVA-OA一致。但当OA浓度为273μmol/L时,其生长抑制作用弱于OVA-OA的。OVA-OA和OA均会促使乳酸脱氢酶（Lactate dehydrogenase,LDH）释放,增大晚期凋亡和坏死的细胞百分比,阻滞细胞周期在G0/G1期和S期。由此可知,OVA-OA对结肠癌细胞具有HAMLET样生长抑制作用,其中OA起主要作用。OVA-OA对人结肠癌细胞的生长抑制作用是通过损伤细胞膜、阻滞细胞生长周期和促进细胞凋亡来实现的。
A complex of α-lactalbumin and oleic acid has previously been shown to have growth-inhibitory effect on cancer cells （Human alpha-lactalbumin made lethal to tumor cells, HAMLET）. Qvalbumin and oleic acid complex was prepared and its cell growth inhibition effect,along with contribution of oleic acid,were all evaluated on Caco-2 cell by using cell-growth inhibition,cellular membrane integrity,cell cycle and apoptosis assays.The result showed that OVA-OA significantly（ p 〈 0.05）inhibited cell growth at 182 μmol/L（ OA）, and the inhibitory effect was enhanced as the concentration was increased to 273 μmol/L.The inhibitory effect was also increased as incubation time was extended in a time-dependent manner. OA at 182 μmol/L also exhibited remarkable cell-growth- inhibitory effect in a similar pattern to OVA-OA,while its inhibitory effect was lower than that of OVA-OA at the same OA concentration of 273 μmol/L. Both OVA-OA and OA could increase LDH release rate, lead to cycle arrested at G0/G1 and S phases,and induce apoptosis.ln conclusion,the complex of OVA and QA has HAMLET- like cell-growth-inhibitory effect,which could be mainly contributed to OA.The mechanisms were associated with damage of cellular membrane integrity,arrest of cell cycle and induction of cell apoptosis.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第18期133-138,142,共7页
Science and Technology of Food Industry
基金
"十二五"农村领域国家科技计划课题子课题(2012BAD28B08)
关键词
卵白蛋白
油酸
细胞生长抑制
乳酸脱氢酶
细胞凋亡
细胞周期
ovalbumin
oleic acid
cell-growth inhibition
lactate dehydrogenase
cell apoptosis
cell cycle
