摘要
探讨了葡萄糖-组胺美拉德产物(MRPs)和组胺(Histamine)对人正常肝细胞(HL-7702)生长抑制和细胞周期可能的作用和影响。用不同剂量的葡萄糖-组胺美拉德产物和组胺处理HL-7702细胞,用MTT方法分别检测美拉德产物和组胺对HL-7702细胞的生长抑制效果;用Annexin检测法,在流式细胞仪下观察两种药物对HL-7702细胞周期分布的影响。MRPs和组胺作用于HL-7702细胞24、48、72 h后,对细胞均有生长抑制作用,其抑制作用呈现剂量和时间依赖性。但组胺对HL-7702细胞的生长抑制作用明显强于MRPs;MRPs产物和组胺两种药物作用于细胞72 h后对细胞周期都有一定的影响,其中组胺的作用明显强于MRPs。葡萄糖和组胺生成的产物能显著消减组胺对HL-7702细胞的杀伤作用。
The effects of Maillard reaction products(MRPs) and histamine on the inhibition of cell proliferation and cell cycle of human HL7702 were investigated. Human HL 7702 cells were respectively treated by MRPs and histamine with different concentrations for 24,48 and 72 h,i.e.,0.2 mg/m L、0.4 mg/m L、0.6 mg/m L、0.8 mg/m L、1 mg/m L for MRPs,and 0.047 mg/m L、0.094mg/m L、0.141 mg/m L、0.188 mg/m L、0.235 mg/m L for histamine. The inhibition of cell proliferation induced by MRPs and histamine was detected by MTT assay. The effects of MRPs and histamine on the cell cycle distribution in human HL7702 were detected with flow cytometry using Annexin assay.The inhibition of cell proliferation induced by MRPs and histamine was dependent on the treatment concentration and duration,in which the inhibition was observed after 24 h、48 h、72 h treatment in human HL7702. Histamine was a stronger inhibitor compared with MRPs. The cell cycle arrest was affected by both MRPs and histamine,while histamine was more effective. In conclusion,MRPs could significantly reduce the histamine-induced cytotoxicity of human HL-7702 cells.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第7期704-711,共8页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31201284
81102121)
宁波市农业创新创业重点项目(2012C92016)
海洋公益性行业科研专项(201305013)
浙江省省级公益性技术应用研究计划项目(2013C32109)
