摘要
目的：探讨vWF19内含子MspI、FgBβ因启动区-148C/T多态性与冠心病血瘀证的关系。方法：将150例确诊为冠心病的患者分为血瘀证组100例、非血瘀证组50例,另抽取体检健康者50例作为健康对照组。采用聚合酶链-限制性片段长度多态性（PCR-RFLP）方法对vWFMspI、FgBβ因启动区-148C/T进行基因多态性分析。结果：与健康对照组相比,vWF内含子MspI基因型与等位基因分布频率在冠心病血瘀证组差异有统计学意义（P〈0.01）,而在非血瘀证组的分布差异则无统计学意义（P〉0.05）;在冠心病血瘀证与非血瘀证组间的分布差异亦无统计学意义（P〉0.05）。M-M-基因型患冠心病血瘀证的OR值为2.970（1.470-6.000,P〈0.01）,而患冠心病非血瘀证的OR值为2.077（0.934-4.615,P〉0.05）;M-等位基因患冠心病血瘀证的相对危险度为1.888（1.082-3.296,P〈0.05）。与健康对照组相比,FgBβ148C/TT基因型与等位基因分布频率在冠心病血瘀证组差异有统计学意义（P〈0.05）,而在非血瘀证组的分布差异则无统计学意义（P〉0.05）;在冠心病血瘀证组与非血瘀证组间的分布差异亦无统计学意义（P〉0.05）。TT基因型罹患冠心病血瘀证与冠心病非血瘀证的相对危险度分别为3.244、2.524,p值分别为0.033、0.137;而T等位基因罹患冠心病血瘀证组与非血瘀证的相对危险度分别为12.317、2.244,p值分别为0.000、0.015。结论：vWF基因MspI多态性中,M-M-基因型与M-等位基因与冠心病血瘀证发生相关,且可能为其独立危险因素;Fg基因-148多态性中,TT基因型与冠心病血瘀证发生相关,T等位基因可能与冠心病发生相关。
Objective： To investigate the association between the polymorphisms of vWF19 MspI and FgBβ148 C / T and blood stasis syndrome of coronary heart disease（ CHD）. Methods： A total of 150 patients with a confirmed diagnosis of CHD were divided into blood stasis syndrome group（ 100 patients） and non- blood stasis syndrome group（ 50 patients）,and another 50 healthy persons who underwent physical examination were enrolled as healthy control group. Polymerase chain reaction- restriction fragment length polymorphism was used to analyze the polymorphisms of vWF MspI and FgBβ 148 C / T. Results： There were significant differences in the frequencies of vWF MspI alleles and genotypes between the blood stasis syndrome group and the healthy control group（ P〈0. 01）,while there were no significant differences between the non- blood stasis syndrome group and the healthy control group（ P〉0. 05）,as well as between the blood stasis syndrome group and the non- blood stasis syndrome group（ P〉0. 05）. The odds ratio（ OR） for the patients with M-M-genotype to develop blood stasis syndrome of CHD was 2. 970（ 1. 470- 6. 000,P〈0. 01）,and the OR for these patients to develop non- blood stasis syndrome of CHD was 2. 077（ 0. 934- 4. 615,P〉0. 05）. The relative risk for the patients with M-allele to develop blood stasis syndrome of CHD was 1. 888（ 1. 082- 3. 296,P〈0. 05）. There were significant differences in the frequencies of FgBβ 148 C / TT genotype and allele between the blood stasis syndrome group and the healthy control group（ P〈0. 05）,while there were no significant differences between the non- blood stasis syndrome group and the healthy control group（ P〉0. 05）,as well as between the blood stasis syndrome group and the non- blood stasis syndrome group（ P〉0. 05）. The relative risks for the patients with TT genotype to develop blood stasis syndrome or non- develop blood stasis syndrome of CHD were 3. 244 and 2. 524,respectively（ P = 0. 033 and0. 137）,while the
出处
《湖南中医杂志》
2016年第8期1-5,共5页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省中医药科研计划项目(编号:24201)
关键词
冠心病
血瘀证
基因多态性
VWF
FG
coronary heart disease
blood stasis syndrome
gene polymorphism
vWF
Fg
