摘要
目的采用高效液相色谱法对苯扎氯铵中烷基组成的比例进行测定,并比较国内外样品的差异。方法采用Inertsil CN-3色谱柱(4.6 mm×250 mm,5μm),以0.1 mol·L^(-1)醋酸钠溶液(醋酸调节p H至5.0)-乙腈(53∶47)为流动相,流速为2.0 m L·min^(-1),检测波长为254 nm,柱温为35℃。结果 n-C_(12)、n-C_(14)、n-C_(16)分别在0.252~25.20、0.121~12.11、0.018~1.844 mg·m L^(-1)与峰面积呈良好的线性关系,检出限分别为109.6、172.9、120.0 ng,3种单体的3个不同浓度的回收率在97.8%~102.4%,RSD在0.16%~1.2%。结论经方法学验证,本方法能快速、准确测定苯扎氯铵中烷基组成比例。
Objective To determine the alkyl components in benzalkonium chloride by RP-HPLC method and compare domestic and foreign products. Methods Inertsil CN-3 chromatographic column（4.6 mm×250 mm, 5 μm） was used. The mobile phase was 0.1 mol·L（-1） sodium acetate solution（using acetic acid to adjust p H to 5.0）-acetonitrile（53∶47）. The flow rate was 2.0 m L·min（-1）, the detection wavelength was 254 nm. The column temperature was 35 ℃. Results n-C_（12）, n-C_（14） and n-C_（16） showed good linearity at 0.252- 25.20 mg·m L（-1）, 0.121-12.11 mg·m L（-1） and 0.018- 1.844 mg·m L（-1）, respectively. The detection limits were 109.6, 172.9 and 120.0 ng, respectively. Recoveries of 3 homologs at different levels were at 97.8%- 102.4% and the RSD was at 0.16%-1.2%. Conclusion This method can be used to the determine the alkyl compositions in benzalkonium chloride quickly and accurately.
出处
《中南药学》
CAS
2016年第8期859-863,共5页
Central South Pharmacy
关键词
苯扎氯铵
高效液相色谱法
烷基组成比例
benzalkonium chloride
high performance liquid chromatography
alkyl component
