摘要
目的以秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)为对象研究山楂提取物(haworth fruit extract,HFE)延缓衰老作用及其可能的作用机制。方法将秀丽隐杆线虫饲喂于含有不同浓度(0、25、50、100μg/ml)山楂提取物的NGM培养基,用计数法统计线虫的平均、最高寿命及繁殖能力,用荧光法观察脂褐素的自发荧光强度,DCFDA探针法检测ROS水平,试剂盒法测定SOD活力,用PCR法检测抗氧化相关基因mRNA表达量。结果饲喂HFE后,线虫表寿命明显延长,繁殖能力显著下降。线虫的脂褐素自发荧光明显减弱,并且与HFE浓度呈剂量依赖效应。线虫的体内ROS水平也显著减少,50、100μg/ml组线虫体内SOD活力显著提高,let-60、cat-1、daf-16、cdc-42、sir-2.1 mRNA的表达水平显著上调,daf-2 mRNA表达水平显著下调。结论山楂提取物可有效延缓衰老,其机制主要涉及提高线虫抗氧化防御系统功能、调控体内内源性抗氧化基因mRNA的表达水平。
Objective To study the aging-delaying effects of Hawthorn fruit extract （HFE） and its possible mechanism in Caenorhabditis elegans （C. elegans）. Methods The C. elegans were fed a NGM medium with different doses of HFE （0, 25, 50, 100μg/ml） and the impacts on lifespan and reproductive capacity were observed by counting method. Fluorescence intensity of lipofuscin was detected. Reactive oxygen species （ROS） level was examined by DCFDA probe and the SOD activity was determined by a commercial kit. Additionally, PCR method was used to detect antioxidant related genes mRNA expression levels. Results After feeding with different doses of HFE, C. elegans exhibited a longer lifespan and lower reproductive ability than those of the normal group. The lipofuscin autofluorescence level in C. elegans was significantly decreased in a dose-dependent manner. DCFDA probe results showed that the total ROS was significantly reduced after HFE supplementation. The SOD activity was increased significantly after 50, 100p, g/ml HFE supplementations （P〈0.05） and the antioxidant-related genes mRNA expression levels were also affected differently. The mRNA expression levels of let-60, cat-1, daf-16, cdc-42 and sir-2.1 were significantly upregulated while daf-2 mRNA expression was significantly reduced. Conclusion HFE can effectively slow down the ageing process and its mechanism mainly involves the improvement of the antioxidant defense system as well as the regulation of the antioxidant gene mRNA expression levels.
出处
《营养学报》
CAS
CSCD
北大核心
2016年第4期391-396,共6页
Acta Nutrimenta Sinica
基金
国家自然科学(青年)基金项目(No.31201322)
"十二五"国家科技计划项目(No.2012BAD33B05)
天津市高等学校科技发展基金计划项目(No.20100609)
天津科技大学卓越人才实验班专项项目(No.1314A216)
关键词
秀丽隐杆线虫
山楂提取物
抗氧化
衰老
hawthorn fruit extract
Caenorhabditis elegans
antioxidant activity
aging
