摘要
为分析禽呼肠病毒(ARV)标准毒株S1133株感染鸡胚成纤维细胞(CEF)后对相关鸡Toll样受体(ChTLRs)mRNA转录水平的影响作用,利用实时荧光定量PCR,测定和分析ARV-S1133感染CEF后ARV结构蛋白σC和ChTLRs的mRNA转录水平变化情况。结果表明,ARV-S1133感染CEF 10h后,ARVσC蛋白的mRNA相对表达量开始迅速上升,在48h达到峰值;同时,感染CEF中的ChTLR3、ChTLR5、ChTLR7、ChTLR15和ChTLR21mRNA表达量发生显著变化,在感染72h内各个受体的mRNA表达量呈波浪式变化。5个不同滴度的ARV感染CEF 24 h后,ChTLR3、ChTLR5、ChTLR7、ChTLR15、ChTLR21mRNA转录水平与病毒滴度均呈正线性相关。上述结果表明,ARV感染后可诱导CEF ChTLR3、ChTLR5、ChTLR7、ChTLR15、ChTLR21的mRNA转录水平发生变化,可能与禽呼肠病毒的复制和致病机制相关。
In order to analyze the mRNA transcriptic changes of chicken Toll-like receptors in ARV standard strain S1133-infected CEF cells, real-time PCR was used to analyze the mRNA transcriptic changes of ARV structural protein aC and chicken Toll-like receptors in ARV S1133-infected CEF cells. The results showed that the relative expression levels of ARV structural protein σC were elevated since 10 h post-infection, reaching a peak at 48 h postinfectioru During this period, the mRNA transcriptic levels of ChTLR3, ChTLRS, ChTLR7, ChTLR15 and ChTLR21 genes were changed significantly in the infected CEF cells with different patterns. Meanwhile, the results of different ARV dose infection assay showed that the mRNA transcriptic levels of ChTLR3, ChTLRS, ChTLR7, ChTLR15and ChTLR21 genes were positively correlated with the virus doses. These data above suggest that ARV infection could effectively induce the mRNA expression changes of ChTLR3, ChTLRS, ChTLRT, ChTLR15 and ChTLR21. Furthermore, ChTLR3, ChTLR5, ChTLR7, ChTLR15 and ChTLR21 may play important roles in replication and pathogenic mechanisms of ARV.
出处
《动物医学进展》
北大核心
2016年第9期1-5,共5页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31160512)
广西自然科学基金项目(2014GXNSFCA118006)
广西特聘专家专项项目(2011B020)
广西水产畜牧兽医局科技项目(桂渔牧科201452003)
