摘要
为了更好地揭示禽网状内皮组织增生症病毒(REV)感染致瘤及免疫抑制的机制,利用Illumina HiSeq^(TM)技术对REV感染鸡胚成纤维细胞(CEF)后宿主细胞的转录组学进行了全面分析,鉴定REV感染24 h宿主差异表达基因1 147个,感染120 h差异表达基因2 254个。通过差异表达基因GO分析和KEGG分析,发现TLRs、NLRs、RLRs等模式识别受体信号通路、WNT信号通路、JAK-STAT信号通路等在REV的致瘤及免疫调控机制中发挥重要作用,而且REV感染后可以诱发宿主的炎症反应,进一步加强REV的致病性及与其他病原的混合感染。结果对揭示REV的致病、致瘤及免疫抑制的分子机制提供了新的信息具有重要意义。
In order to further reveal the mechanisms of tumorigenicity and immunosuppression caused by reticuloendotheliosis virus（REV） infection, Illumina Hi Seq^（TM） technology was used to conduct a comprehensive analysis of the transcriptional changes of chicken embryonic fibroblast（CEF） infected with REV. There are 1 147 and 2 254 differentially expressed genes were identified in host cells infected with REV after 24 hours post infection（hpi） and 120 hpi,respectively. In GO and KEGG analysis of the differentially expressed genes, the pattern recognition receptors（PRRs）signaling pathways, such as TLRs, NLRs, and RLRs, WNT signaling pathway, and JAK-STAT signaling pathway might play an important role in the tumorigenic and immune regulatory mechanisms of REV. Furthermore, REV infection could induce host′s inflammatory response, which was able to further aggravate the pathogenicity of REV and co-infection with the other pathogens. The data shown in this study provided new information for revealing the molecular mechanism of the pathogenic, tumorigenic, and immunosuppressive effects of REV, and further investigation was needed to reveal the detail molecular mechanism of REV infection.
作者
王静
杨彦超
李凯
高立
王永强
张艳萍
崔红玉
刘长军
祁小乐
高玉龙
潘青
王笑梅
WANG Jing;YANG Yanchao;LI Kai;GAO Li;WANG Yongqiang;ZHANG Yanping;CUI Hongyu;LIU Changjun;QI Xiaole;GAO Yulong;PAN Qing;WANG Xiaomei(State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang 150069;Jiangsu Co-innovation Center for Prevention and Control of Important Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009)
出处
《中国家禽》
北大核心
2018年第14期19-24,共6页
China Poultry
基金
黑龙江省自然科学基金项目(QC2017029)
国家重点研发计划(2016YFD0500800)
关键词
REV
CEF
致瘤
免疫抑制
转录组学分析
REV
CEF
tumorigenicity
immunosuppression
transcriptomic analysis
