摘要
目的通过导向分离法发现姜黄Curcuma longa根茎中1个新化合物,并进行分离鉴定及细胞毒活性研究。方法运用LC-MS分析技术,结合MCI-Gel小孔树脂、ODS-C18反相柱及RP-HPLC等柱色谱手段分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果从姜黄根茎甲醇提取物的低极性流分中分离得到1个姜黄素类新化合物,鉴定为姜黄素-没药烷型倍半萜二聚体化合物3,4′′′-epoxy-5′′′-C-(1α,2β,3β-bisabola-4,10-diene-9-one)-(2→5′′′)-curcumin(1),该化合物对Hep G2、A549和CT-26肿瘤细胞IC50值分别为4.82、8.81和5.40μmol/L。结论化合物1为1个姜黄素类新化合物,命名为姜萜素G;其对肿瘤细胞具有显著的细胞毒活性。
Objective Separation and purification of a new curcuminoid from the rhizome of Curcuma longa by guided separation method. Methods The chemical constituent was separated and purified through various kinds of chromatographic methods,including MCI-Gel, ODS-C18 reverse phase column, and RP-HPLC. The structure was elucidated by physicochemical properties and spectral data. Results A new curcuminoid, 3,4′′′-epoxy-5′′′-C-（1α,2β,3β-bisabola-4,10-diene-9-one）-（2→5′′′）-curcumin（1）, was isolated from the low polar fraction of methanol extracts from the rhizome of C. longa, named as curcuterpene G. This compound exhibited the inhibitory effect to Hep G2, A549, and CT-26 tumor cells with the IC50 values of 4.82, 8.81, and 5.40 μ mol/L,respectively. Conclusion A new curcuminoid is isolated from the rhizome of C. longa by guided discovery method, and it exhibits notable cytotoxic activities.
出处
《中草药》
CAS
CSCD
北大核心
2016年第14期2418-2423,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81302742)
教育部春晖计划项目(13205638)
四川省教育厅重点项目(11ZA005)
中国博士后科学基金(2012M511935)
关键词
姜黄
姜萜素G
姜黄素类
没药烷型倍半萜
导向分离法
细胞毒活性
Curcuma Longa Rhizoma
curcuterpene G
cucurminoids
bisabola-sesquiterpene
guided separation
cytotoxicity
