摘要
目的筛选RA患者外周血中差异性表达的环状RNA（circRNA）并进行生物信息学分析，以探讨外周血circRNA在RA发病中的作用。方法采用circRNA芯片对3例RA患者和3名健康对照的外周血中的circRNA进行筛查，用R软件包对数据进行归一化及后续数据处理，通过Fold Change和P值进行筛选，采用t检验，寻找2组样本间差异表达的circRNA，并对差异表达的circRNA进行生物信息学分析。结果芯片筛查结果提示有36个circRNA在RA组和对照组之间差异有统计学意义，14个。ircRNA下调，22个circRNA上调。对这些circRNA所涉及的基因进行基因本体论（GO）分析，结果提示，这些基因主要参与生物学调控、细胞分化、代谢过程等。对每个差异表达的circRNA的靶miRNA进行预测，其中有1个circRNA（hsa_cire_0005397）预测结果中有1个miRNA（hsa—miR-125a-3p）在文献中得到验证，用CvtoscaDe软件对三者之间的网络关系进行了预测。结论RA患者外周血中的circRNA出现多个差异性表达，circRNA可能参与RA调控机制。
Objective To screen the peripheral blood circRNA differently expressed in patients with rheumatoid arthritis （RA） and to explore the pathogenetic role of peripheral blood cicRNA in RA by analyzing the data with bioinformatics. Methods The study was performed in 3 RA cases and 3 healthy controls, using circRNA microarrays to screen the circRNA in peripheral blood of patients with RA. The data were normalized and analyzed by R soft package, screening by fold change and P value and searching the differently expressed circRNAs between the two samples by t test. Bioinformatics was performed to analyze the differently expressed circRNAs. Results The results from circRNA microarrays revealed that 36 circRNAs were significantly al-tered in RA patients （P 〈0.05） compared with the control group. Among them, 22 were significantly up-regulated, and the other 14 were down-regulated. The GO analysis of the genes involved in the circRNA showed that these genes participated in the progress of biological regulation, cell differentiation, and metabolism. We predicted the target miRNA of all the differently expressed circRNAs, among the results there was a miRNA （hsa-miR-125a-3p） targeted by a circRNA（hsa_circ_0005397） experimentally confirmed by other studies. The relationships among circRNA-miRNA-Gene were predicted by Cytoscape software. Conclusion There are many differently expressed circRNAs in peripheral blood of patients with RA, and the circRNAs maybe involved in the regulatory mechanisms of Rheumatoid Arthritis.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2016年第8期541-546,I0001,共7页
Chinese Journal of Rheumatology
基金
国家自然科学基金（31501902）
河南省科技厅科技攻关项目（142102310050）
河南省教育厅项目（148310010）
信阳师范学院大学生项目科研项目（2014）
