摘要
目的观察山楂总黄酮对人肝细胞(He PG2)低密度脂蛋白受体表达的影响。方法用MTT法筛选出合适的山楂总黄酮作用浓度,用含不同浓度的山楂总黄酮培养液培养人肝细胞,基于细胞免疫技术,用荧光显微镜结合流式细胞术测定人肝细胞膜低密度脂蛋白受体的表达量和表达活性。结果随着山楂总黄酮浓度升高,人肝细胞低密度脂蛋白受体的表达量和表达活性增加。5、15、25μmol/L的山楂总黄酮在每10 000个人肝细胞中的肝细胞膜低密度脂蛋白受体表达量的平均荧光强度分别为11.21、14.39、19.67;每10 000个人肝细胞中的肝细胞膜低密度脂蛋白受体表达活性分别为6.34、10.11、16.77。结论山楂总黄酮能显著增加人肝细胞膜低密度脂蛋白受体的表达。
Objective To investigate the expression of HepG2 low-density lipoprotein（LDL） receptor affected by hawthorn flavones. Methods MTT method was used to screen appropriate hawthorn flavone concentrations for culture of the HePG2 line.The numbers and activity of LDL receptorwere measured by cell immunity technology and flow cytometer technology. Results With the increase in concentration of hawthorn flavones, the expression and activity of HepG2 LDL receptorincreased. With 5-25 μmol/L amount of hawthorn flavone,the geometric mean of fluorescene value of LDL receptors on 10000 HepG2 cells was11.21, 14.39, 19.67 and the activity value was 6.34,10.11,16.77, respectively. Conclusion Hawthorn flavones can up-regulate the expression of LDL receptorson HepG2.
出处
《浙江中西医结合杂志》
2016年第8期717-718,F0004,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
