摘要
目的:研究金丝桃苷(Hyperoside)对胃癌BGC-823细胞的生长、周期及凋亡的作用,并通过检测半胱氨酸天冬氨酸蛋白酶(caspase 3、caspase 8、caspase 9)的活性探讨金丝桃苷诱导胃癌凋亡的可能机制。方法:采用MTT法检测金丝桃苷对胃癌BGC-823细胞的增殖影响;流式细胞技术检测金丝桃苷对胃癌BGC-823细胞周期和凋亡的影响;比色法测定金丝桃苷对胃癌BGC-823细胞凋亡过程中caspase 3、caspase 8和caspase 9活性影响。结果:金丝桃苷处理BGC-823细胞24h和48h后,细胞生长明显抑制,作用24h和48h时其IC50分别为32.14和22.67μg/mL;金丝桃苷处理组与对照组相比,胃癌BGC-823细胞G0/G1期比例明显增加,凋亡细胞比例明显增加;caspase 3、caspase 8和caspase9活性随药物浓度增加而逐渐升高。结论:金丝桃苷能够通过阻断细胞周期、诱导细胞凋亡有效抗胃癌,其诱导肿瘤细胞凋亡机制可能与激活caspase 3、caspase 8和caspase 9活性密切相关。
AIM. To observe the effects of hyperoside on the cell proliferation, cycle and apoptosis of human gastric cancer cell line BGC- 823 and to explore its possible mechanisms. METHODS： The effects of hyperoside on the proliferation of BGC-823 cells were evaluated by MTT assay. Flow cytometry was used to analyze the cell cycle and apoptosis of the cells exposed to hyperoside. The activities of caspase 3, caspase 8 and caspase 9 were examined by spectrophotometry. RESULTS： With the hyperoside incubation for 24 h or 48 h resulted in a significant inhibition of proliferation in BGC-823 cells as a ICs0 of 32.14/lg/mL and 22.67 /lg/mL. After treatment with hyperoside,the cell cycle was suppressed obviously at Go/G1 phase. The apoptotic rates were significantly higher in the cells treated with hyperoside than in the control. Comparing with the control, the activities of caspase 3, caspase 8 and caspase 9 were up-regu- lated in hyperoside group. CONCLUSION： Hyperoside have significant anti-gastric tumor effects by induce cell cycle arrest and apoptosis of BGC-823 cells, and intracelular caspase 3, caspase 8 and caspase 9 are c with the apoptosis. osely associated
出处
《中国临床药理学与治疗学》
CAS
CSCD
2014年第9期979-983,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
