期刊文献+

基于^1H NMR给药赭石后大鼠尿液的代谢组学分析 被引量:4

1 H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats
下载PDF
导出
摘要 基于1H NMR的代谢组学方法结合多变量数据分析方法(主成分分析和偏最小二乘判别分析)对灌胃给药赭石(2,5和10 g/kg体重剂量)的成年Wistar大鼠尿液进行分析,并对大鼠给药前1天、给药后1~5天尿液1H NMR数据进行单变量比较分析,筛选出赭石的潜在特征代谢物,对赭石引起的代谢变化进行研究,为赭石的科学用药提供依据。结果表明,大鼠体内柠檬酸、牛磺酸、肌酸酐、α-酮戊二酸、琥珀酸、二甲基甘氨酸等代谢物浓度发生明显变化,随给药时间的变化出现恢复趋势,且恢复趋势与给药剂量相关,可作为赭石的潜在特征代谢物。给药2,5和10 g/kg体重剂量赭石降低了大鼠机体三羧酸循环能力,影响了能量、肌酸及二甲基甘氨酸的代谢,且10 g/kg体重剂量赭石对大鼠肝功能造成一定影响。 Wistar rats were intragastrically administered with different doses (2, 5 and 10 g / kg body weight) of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2016年第6期857-863,共7页 Chinese Journal of Analytical Chemistry
基金 国家自然科学基金项目(Nos.20975097,21305134)资助~~
关键词 核磁共振 代谢组学 主成分分析 偏最小二乘判别分析 赭石 Nuclear magnetic resonance Metabonomics Principal component analysis Partial least squares-discriminant analysis Haematitum
  • 相关文献

参考文献19

二级参考文献20

  • 1孙瑞元.简捷实用的半数致死量综合计算法[J]药学学报,1963(02). 被引量:1
  • 2Hong M,Jin Y,Mai Y Q.Comp.Biochem.Physiol.B,1992,101(12):35-39. 被引量:1
  • 3Li L,Sun B,Zhang Q J.Ethnopharmacol.,2008,116(3):561-568. 被引量:1
  • 4Goldsmith P,Fenton H,Morris-Stiff G,Ahmad N,Fisher J,Prasad K R.J.Surg.Res.,2010,160(1):122-132. 被引量:1
  • 5Zhang X X,Li Y B,Zhou H F,Fan S M,Zhang Z Z,Wang L,Zhang Y J.J.Pharmaceut.Biomed.Anal.,2014,97(11):151-156. 被引量:1
  • 6Lu X Y,Tian Y,Lian X P,Jin Y C,Jin T T,Zhao Q Q,Hu B,Shen X P,Fan X H.Food Chem.Toxicol.,2014,65(3):343-355. 被引量:1
  • 7Dudka I,Kossowska B,Senhadri H,Latajka R,Hajek J,Andrzejak R,Antonowicz-Juchniewicz J,Gancarz R.Environ.Int.,2014,68(7):71-81. 被引量:1
  • 8Liu D,Islam E,Li T Q,Yang X E,Jin X F,Mahmood Q.J.Hazard Mater.,2008,153(1-2):114-122. 被引量:1
  • 9Li T Q,Di Z Z,Yang X E,Sparks D L.J.Hazard Mater.,2011,192(3):1616-1622. 被引量:1
  • 10Jiang C A,Wu Q T,Zeng S C,Chen X,Wei Z B,Long X X.J.Environ Sci.(China),2013,25(9):1890-1896. 被引量:1

共引文献93

同被引文献35

引证文献4

二级引证文献25

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部