摘要
基于1H NMR的代谢组学方法结合多变量数据分析方法(主成分分析和偏最小二乘判别分析)对灌胃给药赭石(2,5和10 g/kg体重剂量)的成年Wistar大鼠尿液进行分析,并对大鼠给药前1天、给药后1~5天尿液1H NMR数据进行单变量比较分析,筛选出赭石的潜在特征代谢物,对赭石引起的代谢变化进行研究,为赭石的科学用药提供依据。结果表明,大鼠体内柠檬酸、牛磺酸、肌酸酐、α-酮戊二酸、琥珀酸、二甲基甘氨酸等代谢物浓度发生明显变化,随给药时间的变化出现恢复趋势,且恢复趋势与给药剂量相关,可作为赭石的潜在特征代谢物。给药2,5和10 g/kg体重剂量赭石降低了大鼠机体三羧酸循环能力,影响了能量、肌酸及二甲基甘氨酸的代谢,且10 g/kg体重剂量赭石对大鼠肝功能造成一定影响。
Wistar rats were intragastrically administered with different doses (2, 5 and 10 g / kg body weight) of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2016年第6期857-863,共7页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(Nos.20975097,21305134)资助~~
关键词
核磁共振
代谢组学
主成分分析
偏最小二乘判别分析
赭石
Nuclear magnetic resonance
Metabonomics
Principal component analysis
Partial least squares-discriminant analysis
Haematitum