摘要
采用PCR方法克隆大豆光周期GmGBP1基因启动子,构建含有GmGBP1启动子驱动报告基因GUS的融合性表达载体pBI121-pGmGBP1::GUS,通过花序浸泡法,转化野生型拟南芥获得pBI121-pGmGBP1::GUS转基因植株,对T3代转基因拟南芥进行GUS染色,研究了GmGBP1启动子受激素和外源环境诱导的表达规律。结果表明:大豆GmGBP1启动子受水杨酸(SA)和干旱(PEG)以及高温等外源环境的诱导,与生物信息学预测GmGBP1启动子序列存在的逆境胁迫响应元件的结果相一致。
The expression pattern of the GmGBP1 promoter under hormone and environment were obtained. The GmGBP1 promoter was cloned by PCR and the fusion expression vector was constructed, including the GmGBP1 promoter and the reporter gene GUS. The fusion expression vector was used to infect Arabidopsis thaliana, and T3 generation of transgenic Arabidopsis was obtained, then the GUS staining was used to research the expression pattern of the GmGBP1 promoter in Arabidopsis thaliana. The results showed that the expression of the GmGBP1 promoter was regulated by GA, SA, drought and high temperature, consistent with the predictions of GmGBP1 promoter element by bioinformatics analysis. Our experiment provide theoretical basis for the cultivation of resistant crops in the future.
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第4期683-686,共4页
Soybean Science
基金
国家自然科学基金(31271748)
转基因生物新品种培育科技重大专项(2014ZX08004-005)
东北农业大学"青年才俊"项目(14QC23)
黑龙江省自然科学基金(C2015009)
东北农业大学2015年大学生SIPT计划(201510224187)
东北农业大学2016年大学生SIPT计划(201610224141)
黑龙江省教育厅青年学术骨干项目(1253G010)
十二五农村领域国家科技计划课题(2013AA102602)
东北农业大学研究生科技创新基金(yjscx14047)
