摘要
利用NCBI和Phytozome数据库进行GmIDD基因的同源基因检索,获取该基因在大豆不同染色体中5个拷贝的序列信息。通过对功能结构域的分析结果显示GmIDD基因5个拷贝均含有2个C2H2型(72~92 aa、114~142 aa)、2个C2HC型(149~169 aa、176~195 aa)锌指结构域。GmIDD基因cDNA序列全长为1 227 bp,编码408个氨基酸。系统进化树分析结果显示GmIDD基因5个拷贝中除了Glyma.14g10940和Glyma.17G228000外,其它IDD蛋白间进化距离较远,表明不同大豆IDD基因在功能上可能存在一定差异。长短日照处理下GmIDD表达量的分析结果显示GmIDD基因受短日照诱导表达,因此推测GmIDD可能参与大豆开花的调控过程。
In this study, we used the NCBI and Phytozome databases to retrieve the GmlDD gene homologous gene, and ob- tained 5 copies of the gene in different chromosomes of soybean. We analyzed the functional domain of the 5 copies of the GmlDD gene. The results showed that the five copies of GmIDD gene contained two C2H2 type（72 -92 aa, 114 - 142 aa）, two C2HC type（ 149 - 169 aa, 176 - 195 aa） zinc finger domain. The full sequence of GmIDD transcription factor cDNA was 1 227 bp, encoding 408 amino acids. Phylogenetic tree analysis showed that the evolution of other IDD proteins was distant except Glyma. 14g10940 and Glyma. 17G228000 in the five copies of GmIDD, which indicated that there might he some differences in the function of different soybean IDD genes. The results of GmlDD expression by long and short daylight showed that GmlDD gene was induced by short daylight and we speculate that GmlDD may be involved in the regulation of soybean flowering.
出处
《大豆科学》
CAS
CSCD
北大核心
2017年第4期525-530,共6页
Soybean Science
基金
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015003)
国家重点研发计划"七大农作物育种"重点专项(2016YFD0101005)
转基因生物新品种培育科技重大专项(2016ZX08004-005)
黑龙江省自然科学基金(C2015009)
东北农业大学"学术骨干"(16XG03)
东北农业大学2016年大学生SIPT计划(201610224141)
"十二五"农村领域国家科技计划课题(2013AA102602)
