摘要
目的探讨聚合酶链反应(PCR)在衣原体感染、支原体感染中的临床应用价值。方法选自2013年8月至2014年12月入住我院,疑有非淋菌性尿道炎的患者160例作为研究对象,所有患者均接受PCR沙眼衣原体、解脲支原体检测,探讨PCR技术检测衣原体及支原体感染的临床意义。结果 160例患者中,检测结果呈阳性者共计98例,其中,衣原体阳性患者62例,占63.27%;支原体阳性患者47例,占47.96%;衣原体+支原体皆呈阳性者11例,占11.22%;PCR检测的总符合率为78.75%;灵敏度和特异性分别为94.12%和61.33%。结论 PCR检测具有快速、灵敏等特点,积极开展聚合酶链反应技术进行衣原体、支原体检测,对非淋菌性尿道炎的早期诊断和促进康复具有重要意义。
Objective Investigate the clinical value of PCR in mycoplasma or chlamydia infection, and provide reference fpr clinical diagnosis. Methods 160 patients who were suspected with gonococcal or non-gonococcal urethritis were collected, all pa-tients underwent PCR detection of chlamydia trachomatis and ureaplasma urealyticu; analyzed the Results and discussed the clinical value of PCR. Results The test results were positive in 98 cases in total, of which, 62 cases of chlamydia-positive patients, account-ing for 63. 27%;47 cases of mycoplasma-positive patients, accounting for 47. 96%;trachomatis＋mycoplasma were both positive in 11 cases, accounting for 11. 22%;the coincidence rate of PCR were 78. 75%;sensitivity and specificity were 94. 12% and 61. 33%. Conclusion PCR assay has rapid, sensitive and other characteristics, the positive application of this technique in chlamydia and my-coplasma detection is very important in early diagnosis and promote rehabilitation of non-gonococcal urethritis.
出处
《哈尔滨医药》
2016年第3期237-238,共2页
Harbin Medical Journal
关键词
聚合酶链反应
衣原体感染
支原体感染
Polymerase chain reaction
Chlamydia infection
Mycoplasma infections
