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A7琼脂固体和液体培养联合应用检测泌尿生殖道解脲脲原体 被引量:5

Application of A7 Agar Combining with Broth Culture in Detection Ureaplasma Urealyticum in Urogenital Specimens
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摘要 目的评价解脲脲原体(Uu)培养方法的特异性,了解解脲脲原体的耐药性。方法用A7琼脂固体培养基平板分离鉴定法和肉汤稀释法检测2230例临床标本。结果2230例临床标本,固体培养阳性448例,液体培养阳性593例。两者比较(χ2=128.8,P<0.001)差异有显著性。药敏试验四环素类和喹诺酮类药物有较高的耐药性。结论固体培养和液体培养联合应用可提高解脲脲原体检验的特异性,并能了解其药物敏感性,对临床治疗具有重要指导意义。 Objective To evaluate the specificity of detection ureaplasma urealyticum in urogenital specimens, and to detect the resistance of ureaplasma urealyticum. Methods We detected ureaplasma urealyticum in 2 230 urogenital specimens by A7 agar combining with broth culture. Results 2 230 urogenital specimens were tested, 448 positive samples were tested by A7 agar and 593 positive samples were tested by broth culture. Compare them (χ2=128.8, P<0.001), we can find that they have remarkable differences. The resistances of tetracycline and loxacin group were higher than others. Conclusion Combined application of A7 agar and broth culture can improve the specificity of detection ureaplasma urealyticum and examine the resistance. And this plays an important role in clinical treatment.
出处 《中国中西医结合皮肤性病学杂志》 CAS 2005年第1期34-36,共3页 Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
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