摘要
目的探讨用聚合酶链反应(PCR)检测解脲脲原体(Uu)生物群,在临床实际运用的可行性与必要性。方法采用微量肉汤稀释法测定Uu体外抗菌药物敏感性,并以多条带抗原(MB-Ag)基因为靶位,设计分群引物,对Uu菌株进行生物分群检测。结果124株Uu临床菌株中,男性parvo生物群占36.84%(14/38),T960生物群占63.16%(24/38),未检测到两群混合的情况;女性parvo生物群占74.41%(64/86),T960生物群占23.26%(20/86),两群都检测到的占1.62%(2/86)。Uu两大生物群对四环素、多西环素、洛美沙星的耐药率无显著性差异(P值分别为0.1558、1.0000、0.3801),但对红霉素,阿齐霉素耐药率存在显著性差异(P值分别为0.0008和<0.0001)。结论建议临床工作中运用PCR方法检测Uu生物群,便于指导临床治疗。
Objective To investigate the possibility and importance of the application of PCR to detect biovars of Ure- aplasma urealyticum in clinical practice. Methods A broth microdilution method for determination of the MICs of the Ureaplasma species was performed and the clinical isolates were biotyped by polymerase chain reaction（PCR）according to the multiple-banded antigen（Mt3-Ag）gene. Results Among the 124 Uu isolates,fourteen（36.84% ,14/38）strains proved to belong to parvo biovar, twenty-four （ 63.16 % , 24/38 ） was T960 biovar in man ; sixty-four （ 74.41% , 64/86 ） strains proved to belong to parvo biovar, twenty（ 23.26 %, 20/86 ） was T960 biovar in women and two（ i. 62 %, 2/86 ） was both biovars. There were no significant difference in resistance to tetracycline, doxycycline and lomefloxacin of two bivors. However, the number of erythromycin and azithromycin resistant strains of T960 biovar were significantly higher than that of parvo biovar （P = 0.0008 and P 〈 0.0001 respectively） .Conclusion It is suggested to detect and classify biovars of Uu by PCR in clinical practice as a guideline for its treatment.
出处
《中国艾滋病性病》
CAS
2009年第1期44-46,共3页
Chinese Journal of Aids & STD
基金
2007年广东省科技厅科技计划项目资助(2007B030502013)
关键词
解脲脲原体
生物群
聚合酶链反应
Ureaplasma urealyticum （Uu）
Biovars
Polymerase chain reaction （ PCR ）
