摘要
目的建立福州地区健康儿童外周血淋巴细胞亚群及活化的正常参考范围。方法选取福州地区0-18岁健康儿童593例为研究对象,按年龄分为新生儿组（0-28d）、婴儿组（-12个月）、幼儿组（-3岁）、学龄前组（-7岁）、学龄组（-12岁）和少青组（-18岁）,采集外周血以美国BD公司生产的四色荧光抗体进行标记,以FACSCalibur流式细胞仪测定T淋巴细胞亚群CD3＋、CD3^＋CD4^＋、CD3^＋CD8^＋、B淋巴细胞亚群CD3-CD19^＋、NK细胞CD3^-CD16^＋CD56^＋及淋巴细胞活化指标CD4^＋CD38^＋、CD4^＋AntiHLA-DR^＋、CD8^＋CD38^＋、CD8^＋Anti-HLA-DR^＋的百分率和绝对计数,以Multi SET软件获取数据并分析结果,SPSS 10.0软件进行统计分析。以百分位数法,采用95%可信区间确定正常参考值范围。结果除CD3^-CD16^＋56^＋绝对计数在各年龄组间无差异外,淋巴细胞亚群及淋巴细胞活化指标在不同年龄组之间的差异均有统计学意义,表现为CD3^＋绝对计数、CD3^＋CD4^＋、CD4^＋CD38^＋百分率和绝对计数、CD4＋/CD8＋比值随着年龄的增长呈降低趋势。CD4^＋Anti-HLA-DR^＋的百分率随着年龄的增长呈增高趋势。新生儿组CD3^＋、CD3^＋CD4^＋、CD4^＋CD38^＋、CD8^＋CD38^＋的百分率和绝对计数及CD4^＋/CD8^＋比值明显高于其它年龄组,CD3^-CD19^＋、CD8^＋AntiHLA-DR^＋的百分率和绝对计数明显低于其它年龄组。在不同性别之间CD3^＋CD4^＋和CD3^-CD19^＋、CD4^＋CD38^＋、CD4^＋Anti-HLA-DR^＋和CD8^＋CD38^＋的百分率和绝对计数的差异有统计学意义,表现为女童明显低于男童。儿童淋巴细胞亚群绝对计数的参考值高于成人。结论健康儿童淋巴细胞免疫表型的分布存在性别和年龄差别,由于这种差别细微,可以在同一种族同一区域建立统一的正常参考值范围。本研究成功建立福州地区健康儿童淋巴细胞亚群及活化的正常参考范围。
Objective To establish the reference range of peripheral blood Lymphocyte subsets and the main related parame-ters of activation for healthy children in Fuzhou areas. Methods A total of 593 peripheral blood samples were collected from healthy children aged from 0 to 18 years in the study. The included children were divided into newborn group（0-28d） ,infant group （28d-12m）,toddler age group（1-3y）,preschool age group（3-7y）,school age group（7-12y） and adolescence age group（12-18y）. The percentages and absolute counts of lymphocyte subsets of T cell CD3^＋,CD3^＋CD4^＋,CD3^＋CD8^＋,B cell CD3^-CD19^＋,NK cell CD3^-CD16^＋CD56^＋and activated T cells CD4^＋CD38^＋,CD4^＋Anti-HLA-DR^＋,CD8^＋CD38^＋,CD8^＋Anti-HLA-DR^＋were detected by flow cy-tometry with four-color fluorescent monoclonal antibody. MultiSET software was used to collect and analyze data ,and statistical analysis was performed using SPSS 10.0. 95%confidence interval was used to determine normal reference values. Results Except for the absolute counts of CD3-CD16^＋56^＋,percentages and absolute counts of all the lymphocyte subsets and lymphocyte activation index showed significant differences between different age groups. Absolute counts of CD3^＋T cell,lymphocyte percentages and ab-solute counts of CD3^＋CD4^＋,CD4^＋CD38^＋and the ratio of CD4^＋/CD8^＋were declined with age. CD4＋Anti-HLA-DR＋percentage was increased with age. The percentages and absolute counts of CD3^＋,CD3^＋CD4^＋,CD4^＋CD38^＋,CD8^＋CD38^＋and the ratio of CD4^＋/CD8^＋in newborn group showed significantly higher than those in other age groups,while the percentages and absolute counts of CD3^-CD19^＋and CD8^＋Anti-HLA-DR^＋showed significantly lower than those in other age group. The percentages and absolute counts of CD3^＋CD4^＋,CD3^-CD19^＋,CD4^＋CD38^＋,CD4^＋Anti-HLA-DR^＋and CD8^＋CD38^＋showed significant differences between males and fe-males,and those in females were significantly lower than males.
出处
《实验与检验医学》
CAS
2016年第3期298-303,共6页
Experimental and Laboratory Medicine
基金
福州市科技计划项目(编号2013-S-127-4)
