摘要
目的:探讨正常人外周血中自然杀伤T(NKT)细胞(CD3+CD16+CD56+)比例的95%参考值范围。方法:取141名正常成年人和30例肺癌患者外周血,直接双色荧光抗体染色,溶血洗涤后用流式细胞仪检测NKT细胞和T细胞各亚群的数量。分析获取的结果以百分位数计算95%的正常值范围。结果:141名正常人外周血NKT细胞(CD3+CD16+CD56+)的百分率平均数为4.16%,标准差为4.13%,以百分位数法获得95%参考值范围为(0.78~11.71)%;18~30岁组、31~50岁组和51~70岁组的NKT细胞(CD3+CD16+CD56+)比例的平均数分别为4.64%,3.91%和3.57%,差异无统计学意义(P>0.05);30例肺癌患者组与正常组该项指标的差异亦无统计学意义(P>0.05)。同时检测到正常人CD3+细胞、CD3+CD4+细胞、CD3+CD8+细胞、CD3+CD4+/CD3+CD8+比值以及NK细胞(CD3-CD16+CD56+)的比例均与大多数报道的正常值相似。结论:正常人外周血中NKT细胞(CD3+CD16+CD56+)比例的95%参考值范围是(0.78~11.71)%,平均数为4.16%,中位数为2.97%,虽然NKT细胞比例随年龄增加而减少,但两者之间无直线相关关系。未发现肺癌组患者NKT细胞比例与正常人有显著性差异。
Objective：To detect the proportion of natural killer T （NKT） cells （CD3^＋CD16^＋CD56^＋） in peripheral blood among healthy adults by flow cytometry. Methods： The whole peripheral blood of 141 healthy adults and 30 patients with lung cancer were collected and stained with double colored fluorescent antibodies,followed by lysing red blood cells and washing cells. The percentages of NKT cells, T lymphocytes and T cell subsets were measured and analyzed by flow cytometry. The 95% reference range was calculated by percentile. Results：The average of percentage NKT（CD3^＋CD16^＋CD56^＋） cells in the 141 healthy adults＇ peripheral blood was 4. 16% ;95% reference range by percentile was from 0.78% to 11.71%. The averages of NKT cells in the age groups of 18 - 30-year, 31 - 50-year and 51 - 70-year were 4.64% , 3.91%, and 3.57% , respectively. There was no significant difference among the three groups （P 〉 0.05 ）. The average percentage of NKT cells in the peripheral blood of patients with lung cancer did not change significantly,compared to the normal group. In addition, the proportions of T cell subsets and NK cells measured in this study were within the normal ranges of clinical reference. Conclusions：The reference range of 95% of the NKT cells （CD3^＋CD16^＋CD56^＋） in normal adults＇ peripheral blood is 0.78% to 11.71% ,with an average and median of 4.16% and 2.97% ,respectively. Although NKT cells decrease with the rise of age, there is no linear correlation between them. No significant difference in NKT cell proportions is observed between the lung cancer patients and the normal group.
出处
《蚌埠医学院学报》
CAS
2008年第4期379-382,共4页
Journal of Bengbu Medical College
基金
安徽省教育厅省高校重点实验室重点科研项目(2004Sys008)
